92557-80-7
中文名稱
5-羧基熒光素琥珀酰亞胺酯
英文名稱
5-Carboxyfluorescein N-succinimidyl ester
CAS
92557-80-7
分子式
C25H15NO9
MDL 編號
MFCD00077322
分子量
473.39
MOL 文件
92557-80-7.mol
更新日期
2025/01/03 15:15:26
92557-80-7 結(jié)構(gòu)式
基本信息
中文別名
5-羧基熒光素琥珀酰亞胺酯 英文別名
5(6)-CARBOXYFLUORESCEIN-NHS5(6)-FAM-NHS
5-CARBOXYFLUORESCEIN-NHS
5-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER
5-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER
5-CARBOXYFLUORESCEIN SE
5-CARBOXYFLUORESCEIN SUCCINIMIDE ESTER
5-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER
5-FAM-NHS
5-FAM, SE
1-[[36Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9[9H]xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione
carboxyfluorescein-NHS ester
所屬類別
生物化工：熒光成像物理化學性質(zhì)
熔點>300°C (dec.)
沸點778.7±70.0 °C(Predicted)
密度1.74±0.1 g/cm3(Predicted)
儲存條件−20°C
溶解度堿性溶液（微溶）、DMSO（微溶）、甲醇（微溶）
酸度系數(shù)(pKa)9.31±0.20(Predicted)
形態(tài)固體
顏色黃色到橙色
BRN7865828
InChIKeyGECIDMICWWDIBO-UHFFFAOYSA-N
CAS 數(shù)據(jù)庫92557-80-7(CAS DataBase Reference)
常見問題列表
生物活性
5-FAM SE 是一種單一異構(gòu)體，是一種用于標記肽，蛋白質(zhì)和核苷酸的熒光標記試劑。5-FAM SE 可以與胺反應，并可以產(chǎn)生穩(wěn)定的胺結(jié)合物。體外研究
The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT.
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI).