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92-32-0

中文名稱 焦寧Y(請核實(shí)!)
英文名稱 PYRONIN Y
CAS 92-32-0
分子式 C17H19ClN2O
分子量 302.8
MOL 文件 92-32-0.mol
更新日期 2024/11/04 17:04:01
92-32-0 結(jié)構(gòu)式 92-32-0 結(jié)構(gòu)式

基本信息

中文別名
焦寧Y
吡羅紅Y
哌咯寧Y
派若寧Y
派洛寧Y
派洛寧G
吡羅紅 G
吡啰紅Y(G)
二苯氧雜已胺G
派洛寧Y/吡羅紅G
英文別名
CI 45005
pyronine
PYRONIN G
PYRONIN J
PYRONIN Y
PYRONIN GS
PYRONINE Y
PYRONINE G
pyroninezh
CI NO 45005
所屬類別
生物化工:生物染料

物理化學(xué)性質(zhì)

熔點(diǎn)250-260 °C(lit.)
熔點(diǎn)250-260 °C(lit.)
儲存條件room temp
儲存條件Store at RT.
溶解度易溶于水;微溶于乙醇、乙二醇、甲基溶纖劑
色指數(shù)45005
形態(tài)粉末/固體
顏色粉色
水溶解性Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
最大波長(λmax)548 nm
Merck13,8097
BRN3795789
穩(wěn)定性穩(wěn)定的。與強(qiáng)氧化劑不相容。
生物領(lǐng)域應(yīng)用Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides
InChIKeyINCIMLINXXICKS-UHFFFAOYSA-M
CAS 數(shù)據(jù)庫92-32-0
EPA化學(xué)物質(zhì)信息Pyronine Y (92-32-0)

安全數(shù)據(jù)

危險(xiǎn)性符號(GHS)GHS hazard pictograms
GHS08
警示詞危險(xiǎn)
危險(xiǎn)性描述H341b
危險(xiǎn)品標(biāo)志Xn,Xi
危險(xiǎn)類別碼68-36/37/38
安全說明22-24/25-36-26
安全說明22-24/25-36-26
WGK Germany3
WGK Germany3
RTECS號BQ1450000
TSCAYes
海關(guān)編碼32041300

應(yīng)用領(lǐng)域

用途1
一種插入RNA的陽離子染料,在電泳過程中呈紅色帶。
吡啰紅Y價(jià)格(試劑級)
報(bào)價(jià)日期產(chǎn)品編號產(chǎn)品名稱CAS號包裝價(jià)格
2024/08/1941863派洛寧Y
Pyronin Y, pure, certified
92-32-01g410元
2024/08/1920010派洛寧Y
Pyronin Y, pure, high purity biological stain
92-32-01g595元
2024/08/1941863派洛寧Y
Pyronin Y, pure, certified
92-32-05g1530元

常見問題列表

生物活性
Pyronin Y (Pyronine G) 是一種可插入 RNA 的陽離子染料,可以靶向包括 RNA,DNA 和細(xì)胞器的細(xì)胞結(jié)構(gòu)。Pyronin Y 與雙鏈核酸(尤其是 RNA) 形成熒光復(fù)合物,可以對細(xì)胞 RNA 進(jìn)行半定量分析。Pyronin Y 可用于鑒定活細(xì)胞中核糖核蛋白復(fù)合物的特定 RNA 亞種。
體外研究

Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).

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