92-32-0
基本信息
吡羅紅Y
哌咯寧Y
派若寧Y
派洛寧Y
派洛寧G
吡羅紅 G
吡啰紅Y(G)
二苯氧雜已胺G
派洛寧Y/吡羅紅G
pyronine
PYRONIN G
PYRONIN J
PYRONIN Y
PYRONIN GS
PYRONINE Y
PYRONINE G
pyroninezh
CI NO 45005
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
報(bào)價(jià)日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價(jià)格 |
2024/08/19 | 41863 | 派洛寧Y Pyronin Y, pure, certified | 92-32-0 | 1g | 410元 |
2024/08/19 | 20010 | 派洛寧Y Pyronin Y, pure, high purity biological stain | 92-32-0 | 1g | 595元 |
2024/08/19 | 41863 | 派洛寧Y Pyronin Y, pure, certified | 92-32-0 | 5g | 1530元 |
常見問題列表
Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).