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79955-27-4

中文名稱(chēng) 5-羧基熒光素二乙酸酯
英文名稱(chēng) 5-CARBOXYFLUORESCEIN DIACETATE
CAS 79955-27-4
分子式 C25H16O9
分子量 460.39
MOL 文件 79955-27-4.mol
更新日期 2024/11/05 14:05:09
79955-27-4 結(jié)構(gòu)式 79955-27-4 結(jié)構(gòu)式

基本信息

中文別名
5-羧基熒光素 二醋酸
5-羧基熒光素二乙酸酯
5-羧基熒光素雙醋酸鹽
5-羧基熒光素二醋酸鹽
5-CARBOXYFLUORESCEIN DIACETATE 5-羧基熒光素二醋酸鹽
英文別名
CFDA
5-FAM DA
5-diacetate FAM
5(6)-FAM DIACETATE
5-Carboxyfluorescein diacetate
5-carboxy' Uorescein Diacetate
CARBOXYFLUORESCEIN DI-O-ACETATE
5-Carboxyfluorescein Diacetate >
5(6)-CARBOXY-DI-O-ACETYLFLUORESCEIN
5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE
所屬類(lèi)別
有機(jī)原料:羧酸類(lèi)化合物及衍生物

物理化學(xué)性質(zhì)

熔點(diǎn)>250°C
沸點(diǎn)693.1±55.0 °C(Predicted)
密度1.57±0.1 g/cm3(Predicted)
儲(chǔ)存條件2-8°C
溶解度DMSO: soluble
酸度系數(shù)(pKa)6.4(at 25℃)
形態(tài)固體
顏色無(wú)色固體
酸堿指示劑變色ph值范圍Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
最大波長(zhǎng)(λmax)<300nm
BRN68265
生物領(lǐng)域應(yīng)用Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity
主要應(yīng)用Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs

應(yīng)用領(lǐng)域

用途1
一種重要的染色劑,滲透完整細(xì)胞膜的非熒光疏水劑。在細(xì)胞中,5- 羧基熒光素二乙酸酯被酶水解成親水劑。

安全數(shù)據(jù)

危險(xiǎn)品標(biāo)志T
危險(xiǎn)類(lèi)別碼45-46-23/24/25-36/37/38
安全說(shuō)明53-22-26-36/37/39-45
WGK Germany3
海關(guān)編碼32049010

常見(jiàn)問(wèn)題列表

生物活性
5-CFDA(5-Carboxyfluorescein diacetate)是一種可滲入細(xì)胞并被水解為5-carboxyfluorescein,用于標(biāo)記人椎間盤(pán)細(xì)胞以便體外熒光顯微鏡觀察。
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