| Identification | Back Directory | [Name]
5(6)-CARBOXYFLUORESCEIN DIACETATE | [CAS]
79955-27-4 | [Synonyms]
CFDA
5-FAM DA
5-diacetate FAM
5(6)-FAM DIACETATE
5-carboxy' Uorescein Diacetate
5-Carboxyfluorescein diacetate
CARBOXYFLUORESCEIN DI-O-ACETATE
5-Carboxyfluorescein Diacetate >
5(6)-CARBOXY-DI-O-ACETYLFLUORESCEIN
5-(AND-6)-CARBOXYFLUORESCEIN DIACETATE
5-CFDA [5-Carboxyfluorescein diacetate]
5-Carboxy-di-O-acetylfluorescein, 5-CFDA
5-Carboxyfluorescein diacetate≥ 95% (HPLC)
BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC)
5-Carboxyfluorescein Diacetate - CAS 79955-27-4 - Calbiochem
3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid
3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic Acid
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo- | [Molecular Formula]
C25H16O9 | [MDL Number]
MFCD00467494 | [MOL File]
79955-27-4.mol | [Molecular Weight]
460.39 |
| Chemical Properties | Back Directory | [Melting point ]
>250°C | [Boiling point ]
693.1±55.0 °C(Predicted) | [density ]
1.57±0.1 g/cm3(Predicted) | [storage temp. ]
2-8°C
| [solubility ]
DMSO: soluble
| [form ]
Solid | [pka]
6.4(at 25℃) | [color ]
Colorless solid | [PH Range]
Weak green ' uorescence (6.0) to strong green ' uourescence (7.2) | [λmax]
<300nm | [BRN ]
68265 | [Biological Applications]
Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity | [Major Application]
Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs |
| Hazard Information | Back Directory | [Uses]
5-Carboxyfluorescein diacetate is used in the synthesis of Rhodamines. | [Uses]
Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. |
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