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7385-67-3

中文名稱 尼羅紅
英文名稱 NILE RED
CAS 7385-67-3
EINECS 編號(hào) 230-966-0
分子式 C20H18N2O2
MDL 編號(hào) MFCD00011639
分子量 318.37
MOL 文件 7385-67-3.mol
更新日期 2024/12/17 13:43:12
7385-67-3 結(jié)構(gòu)式 7385-67-3 結(jié)構(gòu)式

基本信息

中文別名
尼羅絲
尼羅紅
英文別名
9-(DIETHYLAMINO)-5H-BENZO[A]PHENOXAZIN-5-ONE
NILE BLUE A OXAZONE
NILE RED
9-(diethylamino)-5h-benzo[a]phenoxazin-5-on
nile
9-(diethylamino)benzo[a]phenoxazin-5(5H)-one
FLUORESCENCE QY-STANDARD E CERTIFIED
Fluorescent Spectral Standard E, certified
9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on
Nile Red, pure, 99%
9-Diethylamino-benzo[a]phenoxazin-5-one
7-(Diethylamino)-1,2-[1,3]butadieno-3H-phenoxazine-3-one
9-Diethylamino-5H-benzo[a]phenoxazine-5-one
Oxazine O-17M
Phenoxazone
所屬類別
化學(xué)試劑:多環(huán)化合物

物理化學(xué)性質(zhì)

熔點(diǎn)203-205 °C(lit.)
熔點(diǎn)203-205 °C(lit.)
沸點(diǎn)457.53°C (rough estimate)
密度1.1871 (rough estimate)
折射率1.5700 (estimate)
儲(chǔ)存條件2-8°C
儲(chǔ)存條件room temp
溶解度在甲醇中可溶1mg/mL
酸度系數(shù)(pKa)4.08±0.20(Predicted)
形態(tài)粉末
顏色深紅色至栗色
BRN279110
穩(wěn)定性穩(wěn)定的。與強(qiáng)氧化劑不相容。
InChIInChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChIKeyVOFUROIFQGPCGE-UHFFFAOYSA-N
SMILESC12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1
CAS 數(shù)據(jù)庫(kù)7385-67-3(CAS DataBase Reference)

安全數(shù)據(jù)

危險(xiǎn)性符號(hào)(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險(xiǎn)性描述H302-H315-H319-H335
安全說(shuō)明24/25
安全說(shuō)明S24/25
WGK Germany3
WGK Germany3
海關(guān)編碼29349990

應(yīng)用領(lǐng)域

用途1
生化研究

知名試劑公司產(chǎn)品信息

尼羅紅價(jià)格(試劑級(jí))
報(bào)價(jià)日期產(chǎn)品編號(hào)產(chǎn)品名稱CAS號(hào)包裝價(jià)格
2024/11/11XW73856731尼羅紅
nile red;nile blue a oxazone;9-(diethylamino)benzo[a]phenoxazin-5(5h)-one
7385-67-3250MG260元
2024/11/0841571尼羅紅
Nile Red, 99%, pure
7385-67-3100mg1545元
2024/11/0841571尼羅紅
Nile Red, 99%, pure
7385-67-31g6628元

常見(jiàn)問(wèn)題列表

生物活性
Nile Red (Nile Blue A oxazone, Phenoxazone 9)是一種十分有效的熒光染料,可在疏水環(huán)境下檢測(cè)細(xì)胞內(nèi)脂質(zhì)滴 intracellular lipid droplets。Nile Red 可用于細(xì)胞內(nèi)脂質(zhì),蛋白質(zhì)的疏水域和溶酶體磷脂內(nèi)含體的染色。
靶點(diǎn)
TargetValue
lipid droplet
()
體外研究

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.

體內(nèi)研究

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.

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