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53518-15-3

中文名稱 香豆素 151
英文名稱 Coumarin 151
CAS 53518-15-3
EINECS 編號(hào) 258-599-1
分子式 C10H6F3NO2
MDL 編號(hào) MFCD00006858
分子量 229.16
MOL 文件 53518-15-3.mol
更新日期 2024/12/19 10:42:39
53518-15-3 結(jié)構(gòu)式 53518-15-3 結(jié)構(gòu)式

基本信息

中文別名
7-氨基-4-三氟甲基香豆素
香豆素 151
7-氨基-4-氟甲醇
香豆素490
英文別名
7-AMINO-4-(TRIFLUOROMETHYL)-2H-CHROMEN-2-ONE
7-AMINO-4-TRIFLUOROMETHYL-CHROMEN-2-ONE
7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN
AFC
AKOS B000459
BUTTPARK 20\02-79
COUMARIN 151
7-amino-4-(trifluoromethyl)-2h-1-benzopyran-2-on
7-amino-4-(trifluoromethyl)-2-benzopyrone
Coumarin 490
7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, FOR FLUORESCENCE
7-AMIDO-4-TRIFLUOROMETHYLCOUMARIN
7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, 99+ %
Caspase 8 Fluorometric Substrate, IETD-AFC (FLICE, MASH, Mch5, N-acetyl-Asp-Glu-Val-Asp-AFC
Coumarin 151, laser grade, pure, 99%
7-Amino-4-(trifluoromethyl)coumarin 99%
Coumarine151
7-Amino-4-(trifluoromethyl)-2H-1-benzopyran-2-one
Coumarin 490. Coumarin 15
COUMARIN 151, 99%, LASER GRADE, PURE
所屬類別
生物化工:熒光成像

物理化學(xué)性質(zhì)

熔點(diǎn)221-222 °C(lit.)
沸點(diǎn)314.6±42.0 °C(Predicted)
密度1.4096 (estimate)
儲(chǔ)存條件under inert gas (nitrogen or Argon) at 2–8 °C
酸度系數(shù)(pKa)1.12±0.20(Predicted)
形態(tài)粉末
顏色黃色
水溶解性Soluble in dimethyl sulfoxide (25 mg/ml), dimethyl formamide (25 mg/ml), and methanol (25 mg/ml). Insoluble in water.
檢測(cè)方法HPLC
BRN4456797
CAS 數(shù)據(jù)庫(kù)53518-15-3(CAS DataBase Reference)

安全數(shù)據(jù)

危險(xiǎn)性符號(hào)(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險(xiǎn)性描述H315-H319-H335
危險(xiǎn)品標(biāo)志Xi,Xn
危險(xiǎn)類別碼R36/37/38-R20/21/22
安全說(shuō)明S26-S36-S37/39
WGK Germany3
Hazard NoteIrritant
TSCAT
危險(xiǎn)等級(jí)IRRITANT
海關(guān)編碼29322090

應(yīng)用領(lǐng)域

用途1
適于用作激光染料
用途2
可用作激光染料和蛋白酶的熒光標(biāo)記。

上下游產(chǎn)品信息

香豆素 151價(jià)格(試劑級(jí))
報(bào)價(jià)日期產(chǎn)品編號(hào)產(chǎn)品名稱CAS號(hào)包裝價(jià)格
2024/11/08A184007-氨基-4-三氟甲基香豆素
7-Amino-4-(trifluoromethyl)coumarin
53518-15-30.25g332元
2024/11/08A184007-氨基-4-三氟甲基香豆素, 99%
7-Amino-4-(trifluoromethyl)coumarin, 99%
53518-15-31g982元
2024/11/08XW0253518153047-氨基-4-三氟甲基香豆素53518-15-35G479元

常見(jiàn)問(wèn)題列表

生物活性
7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) 是熒光標(biāo)記物,可用來(lái)靈敏的檢測(cè)蛋白酶。它的激發(fā)和發(fā)射波長(zhǎng)分別為400和500 nm。
體外研究

Amino acid and peptide derivatives of 7-amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities. Caspase activation is measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-Amino-4-(trifluoromethyl)coumarin (Ac-DEVD-AFC). This substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-Amino-4-(trifluoromethyl)coumarin (AFC). Following cell disruption, released 7-Amino-4-(trifluoromethyl)coumarin is separated on HPLC and detected by fluorescence. The appearance of 7-Amino-4-(trifluoromethyl)coumarin in cells is blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases are responsible for the cleavage. γ-glutamyl-7-amino-4-(trifluoro-methyl)coumarin is synthesized and used as a substrate for fluirimetric assay of γ-gluta-myltranspeptidase. The reaction product 7-amino-4-(trifluoromethyl)coumarin is fluorescent at neutral pH values and with excitation and emission wavelengths of 400 and 490 nm, respectively, concentration is linearly related to fluorescence over the range of 10 to 300 pmol/3 mL reaction mixture.

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