3776-39-4
3776-39-4 結(jié)構(gòu)式
物理化學(xué)性質(zhì)
外觀性狀白色針狀色結(jié)晶，可溶于甲醇、乙醇、DMSO等有機(jī)溶劑，來源于溪黃草。
熔點(diǎn)274-276℃ (Decomposition) (ethanol )
沸點(diǎn)610.3±55.0 °C(Predicted)
密度1.37±0.1 g/cm3(Predicted)
酸度系數(shù)(pKa)12.66±0.60(Predicted)
常見問題列表
生物活性
Enmein 從 I. serra 中分離，具有免疫抑制作用。體外研究
Enmein shows significant inhibitory effect toward K562 with an IC 50 value ranging from 3.2 μg/ml to 8.2 μg/ml. Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml. Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%.
Cell Viability Assay
| Cell Line: | Splenic lymphocyte cells |
| Concentration: | 3.13, 6.25, 12.5, 25 μg/ml |
| Incubation Time: | 48 hours |
| Result: | Exhibited an IC 50 value of 17 μg/ml in splenic lymphocytes. |
Cell Cycle Analysis
| Cell Line: | Splenic lymphocyte cells |
| Concentration: | 5.5 μg/ml, 11 μg/ml, 22 μg/ml |
| Incubation Time: | 48 hours |
| Result: | Caused S stage cells and G2/M stage cells decreased. |
體內(nèi)研究
Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression.
| Animal Model: | Balb/c mice |
| Dosage: | 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg |
| Administration: | Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days |
| Result: | Had inhibitory effect on IL-2 release in blood. |