| Identification | Back Directory | [Name]
TRYPSINOGEN | [CAS]
9002-08-8 | [Synonyms]
TRYPSINOGEN
TRYPSINOGEN FROM BEEF PANCREAS
trypsinogen from bovine pancreas
Trypsinogen >2500 U/mg from bovine
Trypsinogen >2500U/mg from bovine pancreas
Trypsinogen, PMSF treated from bovine pancreas | [EINECS(EC#)]
232-651-3 | [Molecular Formula]
C39H55N9O17 | [MDL Number]
MFCD00466939 | [MOL File]
9002-08-8.mol | [Molecular Weight]
921.9 |
| Hazard Information | Back Directory | [Uses]
Trypsinogen from bovine pancreas is suitable for use in:
- the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy
- tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometer
- the secondary structure analysis of proteins by infrared (IR) spectroscopy
- SDS-PAGE as a molecular weight standard (24kDa)
| [General Description]
Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH2 terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys131 - Ser132 resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His46 and Ser183 at the active site. The pH optimum of trypsin is 7 - 9. | [Biochem/physiol Actions]
Hereditary pancreatitis was shown to be caused by a Arg-His substitution at residue 117 of trypsinogen causing auto-activation of trypsinogen to trypsin. |
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