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ChemicalBook--->CAS DataBase List--->838-07-3

838-07-3

838-07-3 Structure

838-07-3 Structure
IdentificationMore
[Name]

5-Methyl-2'-deoxycytidine
[CAS]

838-07-3
[Synonyms]

2'-DEOXY-5-METHYLCYTIDINE
4-AMINO-1-[(2R,4S,5R)-4-HYDROXY-5-(HYDROXYMETHYL)OXOLAN-2-YL]-5-METHYL-PYRIMIDIN-2-ONE
5-METHYL-2'-DEOXYCYTIDINE
5-METHYL-2'-DEXOYCYTIDINE
5-METHYL-DC
2’-deoxy-5-methyl-cytidin
5-methyldeoxycytidine
Cytidine, 2'-deoxy-5-methyl-
2'-Deoxy-5-methyl-D-cytidine
4-amino-1-[(2R,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidin-2-one
2''-DEOXY-5-METHYLCYTIDINE (5-METHYL-2''-DEOXYCYTIDINE)
1-(2-Deoxy-beta-D-ribofuranosyl)-5-methylcytosine
[EINECS(EC#)]

212-655-1
[Molecular Formula]

C10H15N3O4
[MDL Number]

MFCD00006549
[Molecular Weight]

241.24
[MOL File]

838-07-3.mol
Chemical PropertiesBack Directory
[Melting point ]

217-219 °C(lit.)
[Boiling point ]

492.8±55.0 °C(Predicted)
[density ]

1?+-.0.1 g/cm3(Predicted)
[refractive index ]

44 ° (C=1.4, H2O)
[storage temp. ]

−20°C
[solubility ]

DMSO (Slightly, Heated), Methanol (Slightly, Heated), Water (Slightly)
[form ]

Powder
[pka]

pK1:4.33 (25°C)
[color ]

White to Off-white
[Water Solubility ]

Soluble in water.
[Stability:]

Hygroscopic
[InChI]

InChI=1S/C10H15N3O4/c1-5-3-13(10(16)12-9(5)11)8-2-6(15)7(4-14)17-8/h3,6-8,14-15H,2,4H2,1H3,(H2,11,12,16)/t6-,7+,8+/m0/s1
[InChIKey]

LUCHPKXVUGJYGU-XLPZGREQSA-N
[SMILES]

OC[C@H]1O[C@@H](N2C=C(C)C(N)=NC2=O)C[C@@H]1O
[CAS DataBase Reference]

838-07-3(CAS DataBase Reference)
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

R36/37/38:Irritating to eyes, respiratory system and skin .
[Safety Statements ]

S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
[WGK Germany ]

3
[RTECS ]

HA3860000
[HS Code ]

29389090
Hazard InformationBack Directory
[Description]

5-Methyl-2'-deoxycytidine is a nucleoside analogue that inhibits the synthesis of DNA. 5-Methyl-2'-deoxycytidine prevents methylation of guanine, which disrupts the binding of guanine nucleotide-binding proteins to the dna template and prevents polymerase chain reactions. 2'-Deoxy-5-methylcytidine is also a methyltransferase inhibitor, which means it blocks the enzyme responsible for adding methyl groups to cytosine molecules.
[Chemical Properties]

White Solid
[Uses]

5-Methyl-2'-deoxycytidine is a pyrimidine nucleoside that when incorporated into single-stranded DNA can act in cis to signal de novo DNA methylation. It has been used in epigenetics research to investigate the dynamics of DNA methylation pattern in the control of gene expression.
[Uses]

A Cytidine (C998300) analog and also an isostere of Thymidine (T412000).
[Definition]

ChEBI: 5-methyl-2'-deoxycytidine is a 2'-deoxycytidine.
[General Description]

Giel-Pietraszuk et al. developed a new method for quantification of 5-methyl-2'-deoxycytidine (m5C) in the DNA. The technique is based on the conversion of m5C into fluorescent 3, N4-etheno-5-methyl-2'-deoxycytidine (εm5C) and its identification by reversed-phase high-performance liquid chromatography (RP-HPLC)[1].The measurement of 5-meC and 5-medC in urine may help assess changes in DNA methylation status in the whole body and investigate the DNA demethylation mechanism in vivo. 5-MedC was first detected in urine using an inhibition ELISA method;12 however, because ELISA may suffer from poor selectivity and sensitivity, later urinary measurements of 5-medC were developed mainly based on the chromatographic techniques[2].
[storage]

Store at -20°C
[References]

[1] Ma?gorzata Giel-Pietraszuk. “Quantification of 5-methyl-2’-deoxycytidine in the DNA.” Acta biochimica Polonica 62 2 (2015): 281–6.
[2] Chiung-Wen Hu. “Direct Analysis of 5-Methylcytosine and 5-Methyl-2′-deoxycytidine in Human Urine by Isotope Dilution LC-MS/MS: Correlations with N-Methylated Purines and Oxidized DNA Lesions.” Chemical Research in Toxicology 25 2 (2012): 462–470.
Well-known Reagent Company Product InformationBack Directory
[Sigma Aldrich]

838-07-3(sigmaaldrich)
[TCI AMERICA]

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