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ChemicalBook--->CAS DataBase List--->82473-24-3

82473-24-3

82473-24-3 Structure

82473-24-3 Structure
IdentificationMore
[Name]

3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate
[CAS]

82473-24-3
[Synonyms]

3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANESULFONATE
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANE SULPHONATE
CHAPSO
3-((3-cholamidopropyl)dimethylammonio)-2-hydroxy-
CHAPSO 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate
CHAPSO SIGMAULTRA
CHAPSO ELECTROPHORESIS REAGENT
1-Propanaminium, 2-hydroxy-N,N-dimethyl-3-sulfo-N-3-(3.alpha.,5.beta.,7.alpha.,12.alpha.)-3,7,12-trihydroxy-24-oxocholan-24-ylaminopropyl-, inner salt
CHAPSO,ELECTROPHORESISGRADE
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-2-HYDROXY-1-PROPANESULPHONATE (ULTRAPURE)
3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulphonate (Ultrapure) >99.0%
CHAPSO extrapure
[EINECS(EC#)]

617-338-5
[Molecular Formula]

C32H58N2O5S
[MDL Number]

MFCD00081079
[Molecular Weight]

582.88
[MOL File]

82473-24-3.mol
Chemical PropertiesBack Directory
[Appearance]

White crystalline powder
[Melting point ]

184-186 °C(lit.)
[density ]

1.0453 (rough estimate)
[refractive index ]

1.6900 (estimate)
[storage temp. ]

2-8°C
[solubility ]

H2O: 10 mg/mL at 20 °C, clear, colorless
[form ]

White solid
[color ]

White crystalline
[Odor]

Odorless
[Water Solubility ]

10 g/l at 20 °C
[Merck ]

13,2052
[BRN ]

5842642
[CAS DataBase Reference]

82473-24-3(CAS DataBase Reference)
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

R36/37/38:Irritating to eyes, respiratory system and skin .
[Safety Statements ]

S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
[WGK Germany ]

3
[F ]

3-10
Hazard InformationBack Directory
[Chemical Properties]

White crystalline powder
[Application]

3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
CHAPSO has been used:
in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins
A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.
[Uses]

3-(cholamidopropyl)(dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO) may be used in bicelle preparation for β2-adrenergic receptor reconstitution.
[Uses]

Nondenaturing biological detergent.
[General Description]

Unique detergent with high CMC that solubilizes membrane-bound proteins in their native state. Capable of solubilizing opiate receptor to a state exhibiting reversible binding of opiates. Has higher solubility than CHAPS because of its more polar head group. Micellar average MW: 7000 Aggregation number: 11
[Purification Methods]

Dissolve this zwitterionic detergent (~ 60g) in 40% aqueous MeOH and stir it with the mixed-bed ion-exchange resin RG-501 x 8 (100g, Bio-Rad) until the pH of the supernatant is ~ 7. Filter off the resin and evaporate the filtrate to dryness under reduced pressure. Check for homogeneity by TLC on silica gel G in 95% MeOH/5% NH4OH and visualize the spot with iodine vapour (see CHAPS above). [Hjelmeland et al. Anal Biochem 130 72 1983, Matuo et al. Methods Enzymol 198 155 1991.]
Spectrum DetailBack Directory
[Spectrum Detail]

Chapso(82473-24-3)1HNMR
Chapso(82473-24-3)IR
Well-known Reagent Company Product InformationBack Directory
[Sigma Aldrich]

82473-24-3(sigmaaldrich)
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