| Identification | More | [Name]
Bromothymol Blue | [CAS]
76-59-5 | [Synonyms]
3,3-BIS(3-BROMO-4-HYDROXY-5-ISOPROPYL-2-METHYLPHENYL)-2,1LAMBDA6-BENZOXATHIOLE-1,1(3H)-DIONE
3,3'-DIBROMETHYLMOLSULFONPHTHALEIN
3',3''-DIBROMOTHYMOLSULFONAPHTHALEIN
3',3''-DIBROMOTHYMOLSULFONEPHTHALEIN
3',3'-DIBROMOTHYMOLSULFONEPHTHALEIN
3',3''-DIBROMOTHYMOLSULFONPHTHALEIN
3,3''-DIBROMOTHYMOLSULFONPHTHALEIN
3,3'-DIBROMOTHYMOLSULFONPHTHALEIN
3,3'-DIBROMOTHYMOLSULFOPHTHALEIN
A,A-BIS(6-BROMO-5-HYDROXYCARBACRYL)-A-HYDROXY-O-TOLUENESULFONIC ACID G-SULTONE
BETZ 0417
BROMOTHYMOL BLUE
BROMOTHYMOL BLUE ETHANOL(50)
BROMOTHYMOL BLUE TS
BROMTHYMOL BLUE
BTB
DIBROMOTHYMOL SULFONEPHTHALEIN
DIBROMOTHYMOLSULFOPHTHALEIN
PHENOL, 4,4'-(1,1-DIOXIDO-3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2-BROMO-3-METHYL-6-(1-METHYLETHYL)-
PHENOL, 4,4'-(3H-2,1-BENZOXATHIOL-3-YLIDENE)BIS[2-BROMO-3-METHYL-6-(1-METHYLETHYL)-, S,S-DIOXIDE | [EINECS(EC#)]
200-971-2 | [Molecular Formula]
C27H28Br2O5S | [MDL Number]
MFCD00005872 | [Molecular Weight]
624.38 | [MOL File]
76-59-5.mol |
| Chemical Properties | Back Directory | [Appearance]
Pale purple crystalline powder | [Melting point ]
200-202 °C (lit.) | [density ]
1.4668 (estimate) | [Fp ]
38 °C | [storage temp. ]
Store at RT. | [solubility ]
Solubility Sparingly soluble in water, benzene; soluble in ethanol, ether; insoluble in petroleum ether | [Boiling point ]
614.3±55.0 °C(Predicted) | [form ]
Solid | [pka]
7.0, 7.1(at 25℃) | [color ]
Pink-brown to purple | [Odor]
Characteristic odour | [PH]
pH : 6.0～7.6 | [PH Range]
6(yellow)-7.6(blue) | [Stability:]
Stable. Incompatible with strong oxidizing agents. | [Water Solubility ]
slightly soluble | [λmax]
420nm, 435nm, 620nm | [Merck ]
14,1445 | [BRN ]
373934 | [Major Application]
Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system, paints, toys, cleaning products, detergents, textiles, food freshness sensor, identifying fresh and stale rice,wheat, detecting microorganisms, bacterial growth, psychoactive drug, dental materials | [LogP]
8.600 (est) | [CAS DataBase Reference]
76-59-5(CAS DataBase Reference) | [EPA Substance Registry System]
76-59-5(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xn | [Risk Statements ]
R10:Flammable.
R41:Risk of serious damage to eyes.
R36/37/38:Irritating to eyes, respiratory system and skin .
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S39:Wear eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [RIDADR ]
1170 | [WGK Germany ]
3 | [RTECS ]
SJ450000 | [TSCA ]
Yes | [HazardClass ]
3 | [PackingGroup ]
III | [HS Code ]
29349990 |
| Hazard Information | Back Directory | [Description]
Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods. | [Chemical Properties]
Pale purple crystalline powder | [Uses]
As indicator, pH 6.0 yellow; pH 7.6 blue. | [Uses]
Bromothymol blue is a chemical indicator for weak acids and bases. | [Application]
Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.
Suitable for use:
In preparations of pH indicator solutions.
In phospholipid staining on tissues and cells prepared on microscopy slides. | [Definition]
ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-isopropyl-2-methylphenyl groups. | [Preparation]
Bromothymol blue is synthesized by adding elemental bromine to the thymol blue in a solution of glacial acetic acid.
To prepare a solution that is used as a pH indicator, we should dissolve 0.10 g in an 8.0 cm3 N/50 NaOH and then dilute it with water to 250 cm3. To prepare a solution used as an indicator in volumetric work, we should dissolve 0.1 g in the 100 cm3 of 50% (v/v) ethanol. | [General Description]
Bromothymol blue is a pH indicator which is sparingly soluble in water, benzene but is soluble in ethanol. It is insoluble in petroleum ether. It has its major uses in Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system. It is considered as a toxic reagent. | [Purification Methods]
Dissolved the dye in aqueous 5% NaHCO3 solution and precipitate it from the hot solution by dropwise addition of aqueous HCl. Repeat this until the extinction at max 420nm does not increase. It is an indicator: aqueous solutions are yellow at pH 6.0, and blue at pH 7.6. [Beilstein 19/3 V 461.] |
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