| Identification | More | [Name]
Solvent Black 3 | [CAS]
4197-25-5 | [Synonyms]
2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthyl]azo]perimidine
CERES BLACK BN
CI 26105
CI 26150
CI NO 26150
FAT BLACK HB
LABOTEST-BB LT00451850
SOLVENT BLACK 3
SOLVENT BLACK B
SUDAN BLACK B
2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)-1h-perimidin
2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1h-perimidin
2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-Perimidine
abcolblackx60
acetylatedsudanblackb
aizensotblack6
c.i.solventblack3
fastblackhbn
fatblackhb01
hexatypeblackb | [EINECS(EC#)]
224-087-1 | [Molecular Formula]
C29H24N6 | [MDL Number]
MFCD00006919 | [Molecular Weight]
456.54 | [MOL File]
4197-25-5.mol |
| Chemical Properties | Back Directory | [Appearance]
VERY DARK BROWN TO BLACK FINE CRYSTALLINE POWDER | [Melting point ]
120-124 °C(lit.)
| [Boiling point ]
552.68°C (rough estimate) | [density ]
1.4899 (rough estimate) | [refractive index ]
1.4570 (estimate) | [storage temp. ]
Store at RT. | [solubility ]
Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol | [Colour Index ]
26150 | [form ]
Powder/Solid | [pka]
2.94±0.40(Predicted) | [color ]
Very dark brown to black | [Water Solubility ]
Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. | [λmax]
598 nm, 415 nm | [Merck ]
13,8970 | [BRN ]
723248 | [Stability:]
Light Sensitive | [Biological Applications]
Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening | [CAS DataBase Reference]
4197-25-5(CAS DataBase Reference) | [EPA Substance Registry System]
4197-25-5(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
F,Xi | [Risk Statements ]
R11:Highly Flammable.
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S24/25:Avoid contact with skin and eyes .
S16:Keep away from sources of ignition-No smoking .
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [RTECS ]
SD4431500
| [TSCA ]
Yes | [HazardClass ]
IRRITANT | [HS Code ]
32041900 | [Toxicity]
LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 |
| Hazard Information | Back Directory | [Chemical Properties]
VERY DARK BROWN TO BLACK FINE CRYSTALLINE POWDER | [Uses]
Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing. | [Definition]
ChEBI: A member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carring a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in
rozen sections and for staining of protein bound lipids in paraffin sections. | [Preparation]
aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling. | [Safety Profile]
A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx. | [Staining Procedures]
1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids. | [Properties and Applications]
Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.
|
Standard
|
Light Fastness
|
Heat-resistant(℃)
|
water
|
Sodium Carbonate(5%)
|
Hydrochloric acid(5%)
|
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Melting point
|
Stable
|
|
ISO
|
General
|
126
|
100
|
Insoluble
|
No change
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No change
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