| Identification | More | [Name]
4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE | [CAS]
3459-18-5 | [Synonyms]
4-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE
(4'-NITRO)PHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE
4-NITROPHENYL-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE
4-NITROPHENYL-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOSIDE
4-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOSIDE-(1,5)
4-NITROPHENYL-N-ACETYL-B-D-GLUCOSAMINIDE
4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE
GLCNAC1-B-PNP
P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE
P-NITROPHENYL ACETYL-BETA-D-GLUCOSAMINIDE
P-NITROPHENYL-BETA-D-N-ACETYL-GLUCOSAMINIDE
P-NITROPHENYL N-ACETYL-BETA-D-GLUCOSAMINIDE
PNP-BETA-D-GLCNAC
PNP N-ACETYL-BETA-D-GLUCOSAMINIDE
4'-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside
P-NITROPHENYL-N-ACETYL-B-D-*GLUCOSAMINID E
P-Nitrophenyl-2-Acetamido-2-Deoxy-B-D-
1-(4-Nitrophenyl)-N-acetyl-beta-D-glucosaminide
p-Nitrophenyl 2-Acetamido-2-deoxy-b-D-glucopyranoside
4-nitrophenyl 2-acetamido-2-deoxy-β-d-glucopyranoside | [EINECS(EC#)]
222-398-7 | [Molecular Formula]
C14H18N2O8 | [MDL Number]
MFCD00063696 | [Molecular Weight]
342.3 | [MOL File]
3459-18-5.mol |
| Chemical Properties | Back Directory | [Appearance]
Slightly Off-White Crystalline Solid | [Melting point ]
210-212 °C | [alpha ]
-14～-18°(D/20℃) (c=0,H2O) | [Boiling point ]
477.77°C (rough estimate) | [density ]
1.3038 (rough estimate) | [refractive index ]
1.5110 (estimate) | [storage temp. ]
−20°C
| [solubility ]
H2O: 5 mg/mL, clear, colorless to very faintly yellow
| [form ]
Fine Powder | [pka]
12.76±0.70(Predicted) | [color ]
White to off-white | [Water Solubility ]
water: 5mg/mL, clear, colorless to very faintly yellow | [Usage]
Useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine | [λmax]
400nm(Phosphate buffer sol.)(lit.) | [BRN ]
96193 | [CAS DataBase Reference]
3459-18-5(CAS DataBase Reference) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Safety Statements ]
S22:Do not breathe dust .
S24/25:Avoid contact with skin and eyes . | [WGK Germany ]
3
| [F ]
10-21 | [HS Code ]
29329990 |
| Hazard Information | Back Directory | [Chemical Properties]
Slightly Off-White Crystalline Solid | [Uses]
4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine
| [Uses]
substrate for a-L-fucosidases | [Uses]
Useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine. | [Definition]
ChEBI: An N-acetyl-beta-D-glucosaminide in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group. | [Biological Activity]
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5the pH optimum for the enzyme. |
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