| Identification | More | [Name]
ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT | [CAS]
2183-17-7 | [Synonyms]
1-NAPHTHALENOL DIHYDROGEN PHOSPHATE DISODIUM SALT
1-NAPHTHYL DISODIUM ORTHOPHOSPHATE
1-NAPHTHYL PHOSPHATE DISODIUM SALT
1-NAPHTHYLPHOSPHORIC ACID DISODIUM SALT
ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT
ALPHA-NAP-PHOS 2NA
AURORA KA-1623
DISODIUM 1-NAPHTHYLPHOSPHATE
PHOSPHORIC ACID 1-NAPHTHYL ESTER DISODIUM SALT
SODIUM A-NAPHTHYL ACID PHOSPHATE (DISODIUM)
1-Naphthyl phsophoric acid sodium salt
α-Naphthyl phosphate disodium salt
disodium naphthyl phosphate
A-NAPHTHYL PHOSPHATE DISODIUM
1-NAPHTHYL PHOSPHATE DISODIUM SALT HYDRATE 99%
NAPHTHYLPHOSPHORIDE 2 NA SALT
PHOSPHORIC ACID 1-NAPHTHYL ESTER 2 NA SALT
Disodium 1-Naphthyl Phosphate Hydrate [Substrate for Phosphatase]
α-Naphthyl phosphate disodium salt, Disodium 1-naphthyl phosphate
Disodium 1-naphtyl=phosphate | [EINECS(EC#)]
218-564-3 | [Molecular Formula]
C10H7Na2O4P | [MDL Number]
MFCD00041007 | [Molecular Weight]
268.11 | [MOL File]
2183-17-7.mol |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [F ]
10-21 |
| Hazard Information | Back Directory | [Uses]
1-Naphthyl phosphate disodium salt has been used:
- as a component in the mixture for soaking the chromatography paper for determining acid phosphatase activity
- in the staining of the alkaline phosphatase isoforms in 7.5% non-denaturing polyacrylamide gel
- as a component in the mixture for soaking the chromatography paper for preparing acid phosphomonoesterase-reactive imprinting sheets
- as a component in the staining solution for staining the roots for determining phosphomonoesterase activity
| [Purification Methods]
Purify the salt through an acid ion-exchange column (in H+ form) to give the free acid [1136-89-6], M 224.2, which is obtained by freeze drying and recrystallising from Me2CO/*C6H6, or by adding 2.5volumes of hot CHCl3 (or 20 parts of boiling *C6H6) to a hot solution of 1 part acid and 1.2 parts Me2CO and cooling (m 155-157o, 157-158o). The acid is dissolved in the minimum volume of H2O to which 2 equivalents of NaOH are added and then freeze dried, or by adding the equivalent amount of MeONa in MeOH to a solution of the acid in MeOH and collecting the Na salt, washing with cold MeOH, then Et2O, and drying in a vacuum. [Friedman & Seligman J Am Chem Soc 72 624 1950, Chanley & Feageson J Am Chem Soc 77 4002 1955.] The monosodium salt [1136-89-6] is similarly prepared but by using 1 equivalent of NaOH. The phosphate group is hydrolysed at pH 1.1-5.85 at 70o. [Beilstein 6 IV 4226.] It is a substrate for alkaline phosphatase [Gomori Methods Enzymol 4 381 1957, 128 212 1968], and prostatic phosphatase [Babson Clin Chem 30 1418 1984]. [See p 538 in “Metal-organic Compounds”, Chapter 5, Beilstein 6 IV 4226.] |
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| Company Name: |
Energy Chemical
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| Tel: |
021-021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
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