| Identification | Back Directory | [Name]
Y27632 (hydrochloride) | [CAS]
129830-38-2 | [Synonyms]
Y27632 HCl
Y27632 (hydrochloride)
Y-27632, Dihydrochloride Salt, >99%
trans-4-[(R)-1-Aminoethyl]-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride
(+)-trans-N-(4-Pyridyl)-4-[(R)-1-aminoethyl]cyclohexanecarboxamide dihydrochloride
Y 27632 dihydrochloride
(+)-trans-N-(4-Pyridyl)-4-[(R)-1-aminoethyl]cyclohexanecarboxamide dihydrochloride
trans-4-[(R)-1-Aminoethyl]-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride Y 27632 dihydrochloride | [EINECS(EC#)]
813-428-5 | [Molecular Formula]
C14H21N3O.2(HCl) | [MDL Number]
MFCD03490488 | [MOL File]
129830-38-2.mol | [Molecular Weight]
320.258 |
| Chemical Properties | Back Directory | [Melting point ]
258℃ | [storage temp. ]
2-8°C | [solubility ]
H2O: soluble14mg/mL | [form ]
powder | [color ]
white to beige | [biological source]
synthetic (organic) | [optical activity]
[α]/D +3.0 to +5.0°, c =1.0 in methanol | [Water Solubility ]
H2O: 14mg/mL | [Stability:]
Stable for 2 years from date of purchase as supplied. Solutions in DMSO or methanol may be stored at -20° for up to 3 months. |
| Hazard Information | Back Directory | [Usage]
A cell permeable inhibitor of ROCK-1 and ROCK-2 | [Description]
Y-27632 dihydrochloride (129830-38-2) is a specific inhibitor of ROCK family kinases.1 Enhances stem cell survival and proliferation in culture.2 Y-27632 dihydrochloride significantly improves freeze/thaw survival rate for human embryonic stem cells without influencing morphology, karyotype, cell surface markers, or differentiation potential.3 | [Uses]
A cell permeable inhibitor of ROCK-1 and ROCK-2 | [General Description]
Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential drug for treating acute lung injury. | [Biochem/physiol Actions]
Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. | [storage]
Desiccate at RT | [References]
1) Ishizaki et al. (2000), Pharmacological Properties of Y-27632, a Specific Inhibitor of Rho-Associated Kinases; Mol. Pharmacol., 57 976
2) Gauthaman et al. (2010), Effects of ROCK Inhibitor Y-27632 on Normal and Variant Human Embryonic Stem Cells (hESCs) In Vitro: Its Benefits in hESC Expansion; Stem Cell Rev., 6 86
3) Li et al. (2008), The ROCK Inhibitor Y-27632 enhances the Survival Rate of Human Embryonic Stem Cells Following Cryopreservation; Stem Cells Dev., 17 1079
4) Morel et al. (2018), Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature Y-27632; Mol. Cell. Proteomics, 17 1750 [Focus Citation]
5) Yamazaki et al. (2020) Chromatin condensation retains the osteogenic transcription factor, RUNX2, in the nucleus of human mesenchymal stem cells; J. Biomechanical Science and Engineering 15? 1 [Focus Citation] |
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