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ChemicalBook CAS DataBase List EPA
10417-94-4

EPA synthesis

2synthesis methods
-

Yield:10417-94-4 98%

Reaction Conditions:

with immobilized lipase from Candida Antarctica;water in 1,4-dioxane at 55; for 3 h;Enzymatic reaction;

Steps:

2.2. Preparation of EPA acid

To a solution of the ethyl ester of EPA (200mg, 0.6mmol) in 1.7mL of dioxane, 300μL of distilled water and Novozym 435 (200mg) were added. The mixture was stirred at 55°C and 280rpm in a shaker for 3h. Then, the enzyme was separated by filtration and the solution taken to dryness under reduced pressure. The residue was purified by column chromatography (Si gel 3.9 g, from n-hexane to n-hexane/EtOAc 9:1 containing 0.1% of HCOOH, 200mL) to give pure (TLC analysis) EPA (180mg, 0.59mmol, 98% yield) as a colorless oil.

References:

Sanfilippo, Claudia;Paterna, Angela;Biondi, Daniela M.;Patti, Angela [Bioorganic Chemistry,2019,vol. 93,art. no. 103325]

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