Berberine-Based Hypercrosslinked Polymer: Advanced Solid-Phase Microextraction for High-Throughput Analysis of Aristolochic Acids in Environmental Water and?…
Abstract
In this study, a novel berberine based anion-exchanged hypercrosslinked polymer (BBR-HCPs) was synthesized via Friedel-Crafts alkylation using dimethoxymethane as a cross-linking agent. The proposed BBR-HCPs polymer exhibited excellent adsorption capacities for aristolochic acids (AAs). Moreover, there are various interactions such as hydrophobic, π-π stacking, hydrogen bonding, and electrostatic interactions between BBR-HCPs and AAs. A solid phase microextraction (SPME) coating, prepared with BBR-HCPs as the sorbent and polyacrylonitrile (PAN) as the binder, was applied for the determination of AAs in herbal plants using a 24-well plate for high-throughput, with instrumental analysis carried out via high performance liquid chromatography with diode array detector (HPLC-DAD). The optimized and validated BBR-HCPs-SPME/HPLC-DAD method exhibited excellent linearity over range of 5-400?ng?mL-1 with R2?>?0.9974. Detection limits (S/N?=?3) for AAs were found to be 0.03-0.150?ng?mL-1, with recoveries ranging from 82.6 to 117.8?% and relative standard deviations (RSDs) less than 10.49?%. This study underscores the potential of BBR-HCPs as a promising adsorbent for selective extraction and enrichment of AAs while introducing a reliable novel strategy for monitoring trace amounts of AAs in water and herbal plant samples.