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Chemical Engineering Journal

Chemical Engineering Journal

IF: 13.3
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Avi-tag mediated Phage@gold nanoprobe illuminates bacteria

Published:26 November 2024 DOI: 10.1016/j.cej.2024.157801
Ping Li, Mangmang Shen, Xiang Chen

Abstract

The phage demonstrates specific recognition towards its host bacteria, while the gold nanoparticle (GNP) exhibits a strong surface plasmon resonance (SPR) effect and emits visible fluorescence with dark-field microscope (DFM). Therefore, phage modified with multiple GNPs (phage@GNPs) targeting bacteria enables the visualization of targets under DFM. However, a universal strategy for constructing a stable phage nanoprobe suitable for clinical detection remains a challenge. Herein, we developed a GNP-functionalized phage (phage@GNPs) by displaying an AVI-tag at the N-terminal region of the major capsid protein in S55 (a Salmonella phage). The S55 displaying AVI-tag exhibits equivalent infectivity towards various serotypes of Salmonella as the native S55 and can be further biotinylated through a reaction between lysine residues on the AVI-tag and COOH groups on D-biotin mediated. Consequently, biotinylated S55 was mixed with streptavidin functionalized GNPs to generate the S55@GNPs due to the strong interaction between biotin and streptavidin. Analysis results showed that the obtained S55@GNPs maintained native affinity to host bacteria and better monodispersity and stability than the other four modification and assembly methods for constructing phage@GNPs. Furthermore, the results of simulated and clinical Salmonella samples demonstrated that AVI-tag mediated S55@GNPs probes possess a qPCR-like sensitivity with a detection limit of 1?CFU/μL. Notably, the remarkable AVI-tag mediated phage@GNPs DFM strategy covers an extensive detection range spanning from 1 to 50,000?CFU/μL. Additionally, we employed the AVI-tag mediated assemble of phage@GNPs for phages of Vibrio and Klebsiella. The results showed similar data as S55 for illuminating host cells, suggesting that AVI-tag mediated phage@GNPs assembly is a universal strategy applicable to phage of any other bacteria for illuminating their respective host bacterial under DFM.

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