TUNEL原位細(xì)胞凋亡檢測(cè)試劑盒(POD)

中文名稱：TUNEL原位細(xì)胞凋亡檢測(cè)試劑盒(POD)

英文名稱：In Situ Cell Apoptosis Detection Kit, POD

產(chǎn)品規(guī)格：20次|100次

儲(chǔ)存條件：冷凍（-20℃）

概述

Apoptosis is essential in many physiological processes.Several methods have been described to identify apoptotic cells.Endonucleolysis is considered as the key biochemical event of apoptosis,resulting in double-stranded,low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA.Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction.Terminal deoxynucleotidyl transferase(TdT)can catalyze DIG-dUTP to free 3’-OH DNA ends.The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody,which subsequently associate with streptavidin(SABC).Combined with DAB,apoptotic cells stain yellow analyzed under microscopy.

組份

Component A:Labeling Buffer;Component B:TdT(20X);Component C:DIG-dUTP(20X);Component D:Blocking Reagent;Component E:Biotin-conjugated anti-digoxin antibody;Component F:SABC(100X);Component G:Protein K(200X);Component H:Antibody Dilution Solution;Positive control samples
 

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