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pLVX-AmCyan1-C1 載體,pLVX-AmCyan1-C
  • pLVX-AmCyan1-C1 載體,pLVX-AmCyan1-C

pLVX-AmCyan1-C1 載體

價(jià)格 1000
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-11-15
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產(chǎn)品詳情

中文名稱:pLVX-AmCyan1-C1 載體英文名稱:pLVX-AmCyan1-C
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2024-11-15 pLVX-AmCyan1-C1 載體 pLVX-AmCyan1-C 2ug/1000RMB 1000 常溫運(yùn)輸 99% 質(zhì)粒/載體

pLVX-AmCyan1-C1 載體

質(zhì)粒類型:
慢病毒載體
高拷貝/低拷貝:
高拷貝
啟動(dòng)子:
CMV
克隆方法:
多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:
8757 bp (查看載體序列)
載體標(biāo)簽:
N-AmCyan1
載體抗性:
Ampicillin (氨芐青霉素)
篩選標(biāo)記:
Puromycin (嘌呤霉素)
產(chǎn)品編號(hào)
產(chǎn)品名稱
規(guī)格
價(jià)格
ZY2022
pLVX-AmCyan1-C1
2ug
 
pLVX-AmCyan1-C1 質(zhì)粒圖譜

pLVX-AmCyan1-C1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to AmCyan1, a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (1). Genes cloned into the multiple cloning site (MCS), located at the C-terminal end of the AmCyan1 coding sequence, are expressed as C-terminal AmCyan1 fusion proteins. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the AmCyan1 coding sequence. Lentiviral particles derived from the vectorallow the expression of AmCyan1 fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses AmCyan1, and may be used to produce marker virus to optimize infection protocols.

pLVX-AmCyan1-C1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (2), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-AmCyan1-C1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).

In addition to lentiviral elements, pLVX-AmCyan1-C1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

應(yīng)用

To construct a fusion protein, the gene of interest must be cloned into pLVX-AmCyan1-C1 so that it is in-frame with the AmCyan1 coding sequence. The inserted sequence does not require an initiation codon (ATG) or a stop codon (TAA, TAG, TGA); however, if you don't want to use the stop codons downstream of the MCS (see map), you can add a stop codon to the end of your gene of interest.

The fusion protein is constitutively expressed when pLVX-AmCyan1-C1 is transduced into target cells. Before the vector can be transduced, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotype

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關(guān)鍵字: pLVX-AmCyan1-C1 載體/pLVX-AmCyan1-C1質(zhì)粒

公司簡(jiǎn)介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測(cè)試劑等等
成立日期 2016-08-08 (9年) 注冊(cè)資本 100萬元整
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  • 上海澤葉生物科技有限公司
VIP 5年
  • 公司成立:9年
  • 注冊(cè)資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營(yíng)產(chǎn)品:主營(yíng)產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
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詢盤

pLVX-AmCyan1-C1 載體相關(guān)廠家報(bào)價(jià)

產(chǎn)品名稱 價(jià)格   公司名稱 報(bào)價(jià)日期
詢價(jià)
VIP6年
上海弘順生物科技有限公司
2024-11-16
¥1666
VIP6年
成都彼樣生物科技有限公司
2024-11-15
詢價(jià)
明光市興欣礦業(yè)有限責(zé)任公司
2024-11-15
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