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pDsRed2-1 載體,pDsRed2-
  • pDsRed2-1 載體,pDsRed2-

pDsRed2-1 載體

價格 990
包裝 2ug
最小起訂量 2ug
發(fā)貨地 上海
更新日期 2024-11-08
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產(chǎn)品詳情

中文名稱:pDsRed2-1 載體英文名稱:pDsRed2-
保存條件: 常溫運(yùn)輸純度規(guī)格: 99%
產(chǎn)品類別: 質(zhì)粒/載體
2024-11-08 pDsRed2-1 載體 pDsRed2- 2ug/990RMB 990 常溫運(yùn)輸 99% 質(zhì)粒/載體

pDsRed2-1 載體

質(zhì)粒類型:
熒光蛋白報告載體
克隆方法:
多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:
4107 bp (查看載體序列)
5' 測序引物及序列:
DsRed1-N: 'd[GTACTGGAACTGGGGGGACAG]
載體抗性:
Kanamycin (卡那霉素)
篩選標(biāo)記:
Neomycin (新霉素)
備注:
Red fluorescent protein reporter. Used to monitor transcription from cis-regulatory elements.
產(chǎn)品編號
產(chǎn)品名稱
規(guī)格
價格
ZY1100
pDsRed2-1
2ug
 
pDsRed2-1 質(zhì)粒圖譜
pDsRed2-1 載體圖譜

pDsRed2-1 encodes DsRed2, a DsRed variant engineered for faster maturation and lower non-specific aggregation. Derived from the Discosoma sp. red fluorescent protein (drFP583; 1), DsRed2, like its progenitor DsRed1, contains a series of silent base-pair changes that correspond to human codon-usage preferences for high expression in mammalian cells (2). In addition to these changes, DsRed2 contains six amino acid substitutions: V105A, I161T, and S197A, which result in the more rapid appearance of red fluorescence in transfected cell lines; and R2A, K5E, and K9T, which prevent the protein from aggregating. (DsRed2 may, however, form the same tetrameric structure as DsRed1 [3].) In mammalian cell cultures when DsRed2 is expressed constitutively, red-emitting cells can be detected by fluorescence microscopy within 24 hours of transfection. Large insoluble aggregates of protein, often observed in bacterial and mammalian cell systems expressing DsRed1, are dramatically reduced in cells expressing DsRed2. The faster-maturing, more soluble red fluorescent protein is also well tolerated by host cells; mammalian cell cultures transfected with DsRed2 show no obvious signs of reduced viability—in those cell lines tested, cells expressing DsRed2 display the same morphology (e.g., adherence, light-refraction) and growth characteristics as non-transfected controls.

pDsRed2-1 is a promoterless DsRed2 vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the multiple cloning site (MCS). Sequences upstream of DsRed2 have been converted to a Kozak consensus translation initiation site (4) to increase translation efficiency in eukaryotic cells. SV40 polyadenylation signals downstream of the DsRed2 gene direct proper processing of the 3' end of the DsRed2 mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neor ) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of the cassette expresses kanamycin resistance in E. coli.

載體應(yīng)用

DsRed2 can be used as an in vivo reporter of gene expression. Promoters should be cloned into the pDsRed2-1 MCS upstream from the DsRed2 coding sequence. Without addition of a functional promoter, this vector will not express DsRed2. The recombinant DsRed2 vector can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418 (5).

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關(guān)鍵字: pDsRed2-1 載體/pDsRed2-1 質(zhì)粒

公司簡介

上海澤葉生物科技有限公司是一家服務(wù)于生命科學(xué)領(lǐng)域的高新技術(shù)企業(yè),主要為科研機(jī)構(gòu)、高校、院所及企業(yè)產(chǎn)品開發(fā)研究提供所需要的科研類試劑、耗材、儀器、技術(shù)服務(wù)等。包括分子生物學(xué)、免疫學(xué)、微生物學(xué)、細(xì)胞學(xué)、材料科學(xué),通用試劑、藥物合成試劑、手性化合物、催化劑及配體、分析試劑、生物試劑,檢測試劑等等
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  • 上海澤葉生物科技有限公司
VIP 5年
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  • 注冊資本:100萬元整
  • 企業(yè)類型:貿(mào)易
  • 主營產(chǎn)品:主營產(chǎn)品:ELISA試劑盒、重組蛋白、抗體、生化試劑、標(biāo)準(zhǔn)品、分子生物學(xué)試劑、細(xì)胞生物學(xué)等科研用品
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