Chromatography technique
色譜技術(shù)
His tidine-tagged protein purification
Binding Capacity/ml Chromatography Medium
結(jié)合力/ ml色譜介質(zhì)
Approx. 40 mg histidine-tagged protein
Metal Ion Capacity
金屬離子容量
pH stability, operational
PH穩(wěn)定性operational
注：樹脂可以在不明顯改變功能的情況下操作的pH范圍
pH stability, CIP3
PH穩(wěn)定性 CIP3
注：Ni²?剝離介質(zhì)
Pressure/Flow Specification
壓力/流量規(guī)格
Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Chemical stability
化學(xué)穩(wěn)定性
注：Ni²?剝離介質(zhì)
Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea
absin特供GE health care分裝
Ni Sepharose 6 Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Good choice for purification at a variety of scales.
In process scale applications: This resin is used to purify histidine-tagged (his-tagged) and untagged recombinant proteins even at large scales.
In research scale applications: This resin binds 40 mg his-tag protein/mL resin and is suitable for use in batch mode, with gravity flow, or when scalability is needed.
Meets industrial demands with security of supply, robust performance, and regulatory support.
Available as bulk resin and as prepacked columns for use with manual methods or a chromatography system.
Excellent flow properties provide great performance even at high flow rates.
High binding capacity resin with excellent flow properties
Ni Sepharose 6 Fast Flow (FF) is composed of cross-linked 6% agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.