應(yīng)用：
透射電鏡/掃描透射電鏡、光學(xué)顯微鏡、蛋白雜交、純化his標(biāo)簽蛋白時，識別含目的蛋白的部分等實(shí)驗(yàn)。?
優(yōu)點(diǎn)：
靈敏度高?
探針小，分辨率高，1.8 nm Ni-NTA-納米金比抗體探針小?
高溶解度，穩(wěn)定?
無背景?
永久染色?
5 nm Ni-NTA-納米金在電鏡下可見，不需金增強(qiáng)/銀增強(qiáng)?
使用簡單? 
左：1.8nm Ni-NTA-納米金與his標(biāo)簽蛋白相互作用右：用Ni-NTA-納米金標(biāo)記6xhis腺病毒Knob蛋白，未染色的掃描電鏡照片
left: Structure of Ni-NTA-Nanogold??showing interaction with Interaction with a His-tagged protein;?right: Knob protein from adenovirus cloned with 6x-His tag, labeled with Ni-NTA-Nanogold, column purified from excess gold, and viewed in the scanning transmission electron microscope (STEM) unstained (Full width approximately 245 nm).
上：5nm Ni-NTA-納米金的結(jié)構(gòu)，顯示金屬螯合物與his標(biāo)簽蛋白結(jié)合
下：5nm Ni-NTA-納米金的掃描電鏡圖，平均直徑5.11±0.84nm。
Top: Structure of NTA-Ni(II)-5 nm Nanogold?, showing the binding of the incorporated metal chelate to a His-tagged protein; distance from the gold particle surface to the His tag is estimated to be 1.5 nm.?Above: Transmission electron micrograph of 5 nm NTA Nanogold: average diameter 5.11±0.84nm.
| 品名 | 產(chǎn)品描述 | 貨號 | 規(guī)格 |
| 1.8 nm Ni-NTA-Nanogold? | 比抗體小，更易滲透入組織，定位和檢測細(xì)胞、組織或蛋白復(fù)合物中的his標(biāo)簽蛋白。 | 2080 | 10uM 30ml |
| 5 nm Ni-NTA-Nanogold? | 目標(biāo)量達(dá)一定程度時，無需銀增強(qiáng)/金增強(qiáng)可直接用電鏡觀察 | 2082 | 0.5uM 3ml |
相關(guān)產(chǎn)品：
GoldEnhance LM/Blot (GELM) | 金增強(qiáng)試劑，用于光鏡樣品 | 2112 | Initiator/Moderator/Activator/Buffer
各15 ml（共60 ml，足夠做600張載玻片） |
| GoldEnhance EM(GEEM) | 金增強(qiáng)試劑，用于電鏡樣品 | 2113 | Initiator/Moderator/Activator/Buffer
各2 ml（共8 ml，足夠做200個網(wǎng)格） |
| HQ Silver | 用于納米金的質(zhì)量**的增強(qiáng)試劑，均勻顯影，極好的保持結(jié)構(gòu)，特別適合電鏡，光敏感 | 2012 | Initiator/Moderator/Activator
各15 ml（共45 ml） |
| Li Silver | 納米金的銀增強(qiáng)，用于電鏡、光鏡、凝膠、雜交，光不敏感 | 2013 | Initiator/Enhancer
125 ml （共250 ml） |
References：?
1.?Kollman, J. M.; Zelter, A.; Muller, E. G.; Fox, B.; Rice, L. M.; Davis, T. N., and Agard, D. A.: The Structure of the gamma-Tubulin Small Complex: Implications of Its Architecture and Flexibility for Microtubule Nucleation.?Mol. Biol. Cell,?19, 207-215 (2008).
2. Adami, A.; Garcia-Alvarez, B.; Arias-Palomo, E.; Barford, D., and Llorca, O.: Structure of TOR and its complex with KOG1.?Mol. Cell.,?27509-516 (2007).
3. Balasingham, S. V.; Collins, R. F.; Assalkhou, R.; Homberset, H.; Frye, S. A.; Derrick, J. P., and Tonjum, T.: Interactions between the Lipoprotein PilP and the Secretin PilQ in Neisseria meningitidis.?J. Bacteriol.,?189, 5716-5727 (2007).
4. Jiang, Z. G.; Simon, M. N.; Wall, J. S., and McKnight, C. J.: Structural analysis of reconstituted lipoproteins containing the N-terminal domain of apolipoprotein B.?Biophys. J.,?92, 4097-4108 (2007).
5. Pye, V. E, Beuron, F, Keetch, C. A, McKeown, C, Robinson, C. V, Meyer, H. H, Zhang, X, and Freemont, P. S.: Structural insights into the p97-Ufd1-Npl4 complex.?Proc. Natl. Acad. Sci. USA,?104, 467-472 (2007).
6. Promnares, K.; Komenda, J.; Bumba, L.; Nebesarova, J.; Vacha, F., and Tichy, M.: Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits.?J. Biol. Chem.,?281, 32705-32713 (2006).
7. Collins, R. F.; Beis, K.; Clarke, B. R.; Ford, R. C.; Hulley, M.; Naismith, J. H.; and Whitfield, C.: Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules.?J. Biol. Chem.,281, 2144-2150 (2006).
8. Wolfe, C. L.; Warrington, J. A.; Treadwell, L., and Norcum, M. T.: A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins.?J. Biol. Chem.,?280, 38870-38878 (2005).
9. Bumba, L.; Tichy, M.; Dobakova, M.; Komenda, J., and Vacha, F.: Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged NiNTA Nanogold labeling.?J. Struct. Biol.,?152, 28-35 (2005)
10. Collins, R. F.; Frye, S. A.; Balasingham, S.; Ford, R. C.; Tonjum, T., and Derrick, J. P.: Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ.?J. Biol. Chem.,?280, 18923-18930 (2005).
11. Chatterji, A.; Ochoa, W. F.; Ueno, T.; Lin T., and Johnson, J. E.: A virus-based nanoblock with tunable electrostatic properties.?Nano Lett.,5, 597-602 (2005).
12. Buchel, C.; Morris, E.; Orlova, E., and Barber, J.: Localisation of the PsbH subunit in photosystem II: a new approach using labelling of His-tags with a Ni(2+)-NTA gold cluster and single particle analysis.?J. Mol. Biol.,?312, 371-379 (2001).
13. Hainfeld, J. F.; Liu, W.; Halsey, C. M. R.; Freimuth, P., and Powell, R. D.: Ni-NTA-Gold Clusters Target His-Tagged Proteins.?J. Struct. Biol.,?127, 185-198 (1999).
14. Hainfeld, J. F.; Liu, W.; Joshi, V., and Powell R. D.: Nickel-NTA-Nanogold Binds his-Tagged Proteins.?Microsc. Microanal.,?8,?(Suppl. 2: Proceedings) (Proceedings of Microscopy and Microanalysis 2002);?Voekl, E.; Piston, D.; Gauvin, R.; Lockley, A. J.; Bailey, G. W., and McKernan, S., Eds.; Cambridge University Press, New York, NY, 2002, p. 832CD.
GoldiBlot? HIS Western Blot Kit：Ni-NTA-納米金顆粒
應(yīng)用：
1.蛋白雜交檢測帶his標(biāo)簽的重組蛋白（染色時間1小時）?
2.識別細(xì)胞裂解液或提取物中的his標(biāo)簽蛋白?
3.證實(shí)轉(zhuǎn)染細(xì)胞中his標(biāo)簽蛋白的表達(dá)?
優(yōu)點(diǎn)：
更快更靈敏
低背景?
永久信號?
品名 | 產(chǎn)品描述 | 貨號 | 規(guī)格 |
| GoldiBlot? HIS Western Blot Kit | 用于在蛋白雜交中檢測his重組蛋白的 | 2090 | 15個雜交 |
References：
- Dubendorff, J.; Cruz, M.; Gonzalez, C.; Hainfeld, J.; Liu, W.: Rapid Detection of His-tagged Proteins on Western Blots?Proc. 47th Ann. Mtg., Amer. Soc. Cell Biol.,?47; Pres. # 1918., poster # B265 (2007).