名稱 Tris-硼酸電泳緩沖液（10×TBE）TBE Buffer,10×
規(guī)格 500ml
存儲 Store at RT ,12個月有效。
產品簡介
TBE 緩沖液是生物學中常使用的核酸電泳緩沖鹽溶液，主要用于DNA的瓊脂糖凝膠電泳。TBE的主要成分是Tris-硼酸鹽與EDTA，緩沖能力強，適合較長時間電泳，分辨率較高，電泳小于1kb的片段時分離效果好。TBE 緩沖液中的硼酸成分會影響DNA回收效率以及后續(xù)的酶反應，若要進行DNA片段的瓊脂糖凝膠電泳回收實驗，建議使用TAE 緩沖液。
About TBE Buffer
Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (aroun 1 mM concentration).
Although TBE and TAE are common electrophoresis buffers, there are other options for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE is that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.
關鍵字： Tris-硼酸電泳緩沖液
康迪斯化工（湖北）有限公司是一家立足于生命科學領域有自主知識產權的高新技術企業(yè)，主要從事生物試劑的研發(fā)與生產,并以持續(xù)探索和開發(fā)生命科學技術為動力，致力于為科研工作者提供實用、高效的科研試劑，為生命科學的研究與發(fā)展提供有意義的幫助。