名稱 Tween-20（吐溫-20）

規(guī)格 100mL | 500mL

存儲(chǔ) 室溫保存

產(chǎn)品簡(jiǎn)介 

吐溫-20（Tween-20），又稱聚山梨酯-20（Polysorbate-20），一種來(lái)源于山梨聚糖類酯的吐溫類非離子型表面活性劑，易混溶于水，廣泛應(yīng)用于生物化學(xué)研究中，包括：

1）膜結(jié)合蛋白提取實(shí)驗(yàn)中，常以2%濃度去除膜周邊蛋白；

2）基于膜檢測(cè)的免疫學(xué)實(shí)驗(yàn)中，常以0.05%的濃度用作一種封閉劑；

3）還可以0.005~0.5%的濃度用于裂解哺乳動(dòng)物細(xì)胞。

產(chǎn)品性質(zhì) 

中文別名（Chinese Synonym） 聚山梨酯-20
英文別名（English Synonym） 
Polysorbate 20, Polyoxyethylene (20) Sorbitan Monolaurate, TWEEN® 20; Glycosperse L-20X, Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate, 
PEG(20)sorbitan monolaurate
CAS號(hào)（CAS NO.） 9005-64-5
分子量（Molecular Weight） ~1228
密度（Density） 1.095 g/mL(Lit.)
外觀（Appearance） 透明，黃色至琥珀色黏稠液體
臨界膠束濃度（CMC） 0.06 mM (60 mg/L or 6 × 10-3 mole/L)
比重（Specific Gravity） 1.08-1.13
含水量（Water Content） ≤3%
皂化值（saponification number） 40-50
溶解性（Solubility） 易混溶于水（50mg/ml），混溶于醇類，二氧己環(huán)，乙酸乙酯

注意事項(xiàng) 

1）本品不建議高壓滅菌，建議用0.22μm濾膜過(guò)濾除菌。濾膜除菌前可能需要微熱Tween-20到40°C，且用熱水替換部分Tween-20。

2）為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

Biotechnical applications

In biological techniques and sciences, polysorbate 20 has a broad range of applications. For example, it is used:

as a washing agent in immunoassays, such as Western blots and ELISAs. It helps to prevent non-specific antibody binding. In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreaction, to remove unbound immunologicals, and eventually for incubating solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
to saturate binding sites on surfaces (i.e., to coat polystyrene microplates, generally combined with proteins such as BSA).
to stabilize proteins purified protein derivative (PPD) solution used in skin testing for tuberculosis exposure
as a solubilizing agent of membrane proteins
for lysing mammalian cells, at a concentration of 0.05% to 0.5% v/v, generally combined with other detergents, salts and additives.