5'	?	?	A??	G??	T?↓	A??	C??	T??	?	3'	?
3'	?	?	T??	C??	A?↑	T??	G??	A??	?	5'	?

Thermo Scientific ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer. See

Reaction Conditions for Restriction Enzymes

for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a

FastDigest enzyme

for rapid DNA digestion. Isoschizomers: AssI, BmcAI, ZrmI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features:

? Superior quality—stringent quality control and industry leading manufacturing process
? Convenient color-coded Five Buffer System
? Includes universal Tango buffer for double-digestions
? BSA premixed in reaction buffers
? Wide selection of restriction endonuclease specificities

Applications

? Molecular cloning
? Restriction site mapping
? Genotyping
? Southern blotting
? Restriction fragment length polymorphism (RFLP)
? SNP

Note:
Conditions of low salt, high enzyme concentration, glycerol concentration > 5% or pH > 8.0 may result in star activity. Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.

For methylation sensitivity, refer to product specifications.