Recombinant Human Fibroblast Growth Factor 4 is produced by our E.coli expression system and the target gene encoding Ser54-Leu206 is expressed.
Background
Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.
Known as
Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
關鍵字： Recombinant Human Fibroblast Growth Factor 4/FGF-4;細胞生物學
北京索萊寶科技有限公司是一家集研發(fā)、生產(chǎn)、銷售、服務于一體的高科技生物企業(yè)。技術推廣;技術轉讓；技術咨詢；技術服務；生物試劑技術開發(fā)；銷售生物試劑盒、實驗室專用化學試劑、實驗室專用設備、辦公用品、化工產(chǎn)品（不含危險化學品）、計算機軟件及輔助設備；貨物進出口、技術進出口、代理進出口；委托加工生產(chǎn)生物制品、生化制品、化工產(chǎn)品（不含危險化學品）；生產(chǎn)生物試劑盒；批發(fā)第一類醫(yī)療器械、第二類醫(yī)療器械；批發(fā)第三類醫(yī)療器械。