???????Nucleoside-Diphosphate Kinase,中文名為核苷二磷酸激酶,催化腺苷三磷酸(adeno-sine triphosphate, ATP)和核苷二磷酸(nucleosided iphosphate, NDP)之間的磷酸基團轉移反應來維持生物體細胞內的NTP濃度。本產品為大腸桿菌表達的核苷二磷酸激酶,并經簡單純化獲得的可以確保達到一定酶活力的粗酶液。 ???????由于本產品需新鮮制備,確認訂購后約6個工作日才可以發(fā)貨。個別情況首次制備出現(xiàn)問題,發(fā)貨時間會順延。 ???????本產品可以提供十公斤、百公斤乃至噸級的粗酶液,如有需求請聯(lián)系碧云天。 ???????Nucleoside-diphosphate kinases (NDPKs, also NDP Kinase, (poly)nucleotide kinases and nucleoside diphosphokinases) are enzymesthat catalyze the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. Many NDP serve as acceptor while NTP are donors of phosphate group. The general reaction via ping-pong mechanism is as follows: XDP + YTP ←→ XTP + YDP (X and Y each represent different nitrogenous base). NDPK activities maintain an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when guanosine triphosphate (GTP) produced in the citric acid (Krebs) cycle is converted to adenosine triphosphate (ATP). Other activities include cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor, endocytosis, and gene expression. This product with the indicated enzyme activity was briefly purified from engineered E.coli. 產品詳細信息見下表:
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保存條件: ???????-20℃或更低溫度保存,至少1個月有效。 注意事項: ???????由于本產品需新鮮制備,在您確認訂購后約6個工作日才能發(fā)貨。個別情況首次制備出現(xiàn)問題,發(fā)貨時間會順延。 ???????粗酶液的每次凍融均可能會引起部分失活,所以收到酶液后請根據需要進行分裝,并置于-20℃或更低溫度保存。 ???????隨著保存時間的延長,酶活力會有一定程度的下降,收到產品后應盡快使用。 ???????本產品在生產和保存的過程中可能會有混濁或沉淀產生,融化后混勻即可,不影響正常使用。 ???????本產品僅限于專業(yè)人員的科學研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內。 ???????為了您的安全和健康,請穿實驗服并戴一次性手套操作。
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