價(jià)格 | 詢價(jià) |
包裝 | 5μg |
最小起訂量 | 5μg |
發(fā)貨地 | 上海 |
更新日期 | 2024-11-05 |
中文名稱:Recombinant Human SHH | 英文名稱:Recombinant Human SHH |
有效期: 一年 | 產(chǎn)品類別: 重組蛋白 其他重組蛋白 |
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品包裝 | 產(chǎn)品價(jià)格 |
P7212-5μg | Recombinant Human SHH | 5μg | 464.00元 |
Species | Gene ID | Accession | Source | Length | MW | Tag |
Human | 6469 | Q15465 | E. coli | 176aa | 19.8kDa | - |
About this Antibody | |
Name | Recombinant Human SHH (Recombinant Human Sonic Hedgehog Protein N-Terminus; rHuSHH);重組人音猬因子 |
Synonyms | Shh; HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog; sonic hedgehog homolog; sonic hedgehog protein; TPT; TPTPS |
Purity | >98% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1μg/ml, corresponding to a specific activity of >1.0×103IU/mg. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl. |
Endotoxin | Less than 1EU/μg of rHuSHH as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Sonic Hedgehog (SHH) is one of three proteins of the Hedgehog (Hh) family, which also contains Desert Hedgehog (DHH) and Indian Hedgehog (IHH). The three members share a high degree of amino-acid sequence identity (e.g., SHH and IHH are 93% identical). SHH is expressed in fetal intestine, liver, lung, and kidney, but not in adult tissues. The protein consists of 462a.a. with a 23a.a. signal peptide at N-terminus, and is further cleaved into SHH N-Terminus and C-Terminus. SHH has the most critical roles in development, acting as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. In the absence of Sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. SHH-N retains all known signaling capabilities, and can be lipid-modified without receptor affinity reducing, but has more potent than the unmodified form. The rHuSHH has an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue. |
Amino Acid Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
包裝清單:
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
P7212-5μg | Recombinant Human SHH | 5μg |
P7212-25μg | Recombinant Human SHH | 25μg |
P7212-100μg | Recombinant Human SHH | 100μg |
P7212-1mg | Recombinant Human SHH | 1mg |
- | 說(shuō)明書 | 1份 |
保存條件:
???????-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活,所以首次配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。
注意事項(xiàng):
???????由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
???????本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
???????為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
成立日期 | 2007-07-19 (18年) | 注冊(cè)資本 | 1000.000000萬(wàn)人民幣 |
員工人數(shù) | 500人以上 | 年?duì)I業(yè)額 | ¥ 500萬(wàn)-1000萬(wàn) |
主營(yíng)行業(yè) | 生化試劑,核苷,核苷酸,寡核苷酸,抗體,蛋白組學(xué),生物活性小分子 | 經(jīng)營(yíng)模式 | 試劑 |
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