Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

16000

P05017

E. coli

70aa

7.7kDa

－

About this Antibody

Name

Recombinant Murine IGF-1 (Recombinant Murine Insulin-like Growth Factor-1; rMuIGF-1)；Recombinant Mouse IGF-1 ; 重組小鼠胰島素樣生長因子1

Synonyms

IGF1; IGF-1; Somatomedin A; Somatomedin C; IBP1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; somatomedin-C; Somatomedin-C

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rMuIGF-1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant murine IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature murine IGF-1 shares 94% and 98%a.a. sequence identity with human and rat IGF-1, respectively.

Amino Acid Sequence

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

P6105-10μg

Recombinant Murine IGF-1

10μg

P6105-50μg

Recombinant Murine IGF-1

50μg

P6105-100μg

Recombinant Murine IGF-1

100μg

P6105-1mg

Recombinant Murine IGF-1

1mg

-

說明書

1份