Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

338374

Q8CGK6

E. coli

174aa

19.6kDa

－

About this Antibody

Name

Recombinant Murine IFN-λ3/IL-28B (Recombinant Murine Interferon-λ3/Interleukin-28B; rMuIFN-λ3/IL-28B)；Recombinant Mouse IFN-λ3/IL-28B ; 重組小鼠干擾素λ3/白細胞介素28B

Synonyms

IFN-lambda 3; interleukin-28B; IL28B; IL-28B; IL28C; interferon, lambda 3

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30ng/ml, corresponding to a specific activity of >3.3×10⁴IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuIFN-λ3/IL-28B as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interferon lambda 3 (IFN-λ3), also named as interleukin 28B (IL28B), is a protein encoded by the IL28B gene. IL28B, IL28A and IL29 are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by these three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL-10Rβ) and interleukin 28 receptor alpha (IL-28Rα). IFN-λ3 is a cytokine with immune-modulatory activity. It up regulates MHC class I antigen expression and displays potent antiviral activity and antitumor activity, also plays an important role in Toll-like receptor (TLR)-induced antiviral defense and in the antiviral immune defense in the intestinal epithelium. In individuals with chronic hepatitis C, the presence of specific polymorphisms near this gene has been associated with an increased response in treatment for the condition with interferon and ribavirin. The same polymorphisms were also demonstrated at higher frequencies in individuals infected with hepatitis C who cleared the infection spontaneously, compared to those that developed chronic hepatitis from the condition. Murine IFN-λ3 shares 63% amino acid sequence identity with human IFN-λ3.

Amino Acid Sequence

DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6146-5μg

Recombinant Murine IFN-λ3/IL-28B

5μg

P6146-20μg

Recombinant Murine IFN-λ3/IL-28B

20μg

P6146-100μg

Recombinant Murine IFN-λ3/IL-28B

100μg

P6146-1mg

Recombinant Murine IFN-λ3/IL-28B

1mg

-

說明書

1份