Species

Gene ID

Accession

Source

Length

MW

Tag

Porcine

396868

P26891

E. coli

134aa

15.2kDa

－

About this Antibody

Name

Recombinant Porcine IL-2 (Recombinant Porcine Interleukin-2; rPoIL-2)；重組豬白細(xì)胞介素2

Synonyms

aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rPoIL-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL2Rα, 75kDa IL2Rβ, 64kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant porcine IL-2 is a 15.3kDa protein containing 134 amino acid residues and it shares about 72% amino acid sequence identity with mouse, human and rat IL-2. It also shares 60% and 67% acid sequence identity with rhesus macaque and equus caballus IL-2, respectively.

Amino Acid Sequence

APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT

產(chǎn)品編號(hào)

產(chǎn)品名稱(chēng)

包裝

P6461-2μg

Recombinant Porcine IL-2

2μg

P6461-10μg

Recombinant Porcine IL-2

10μg

P6461-100μg

Recombinant Porcine IL-2

100μg

P6461-1mg

Recombinant Porcine IL-2

1mg

-

說(shuō)明書(shū)

1份