Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

60584

O88211

E. coli

180aa

19.8kDa

－

About this Antibody

Name

Recombinant Rat LIF (Recombinant Rat Leukemia Inhibitory Factor; rRtLIF)；重組大鼠白血病抑制因子

Synonyms

CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); Melanoma-derived LPL inhibitor; MLPLI

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Test in process.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μM filtered concentrated solution in 1X PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rRtLIF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature rat LIF shares 91% and 82%a.a. sequence identity with murine and human LIF, respectively.

Amino Acid Sequence

SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF

產(chǎn)品編號(hào)

產(chǎn)品名稱

包裝

P6339-5μg

Recombinant Rat LIF

5μg

P6339-25μg

Recombinant Rat LIF

25μg

P6339-100μg

Recombinant Rat LIF

100μg

P6339-1mg

Recombinant Rat LIF

1mg

-

說(shuō)明書

1份