TFPI-2產(chǎn)品信息

英文名稱：DNA-(apurinic or apyrimidinic site) lyase

中文名稱：DNA-(無嘌呤或無嘧啶位點(diǎn))裂合酶

靶點(diǎn)別稱：TFPI2,PP5,REF1

物種：Human

標(biāo)簽：His Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TFPI-2, His Tag (TF2-H5227) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Lys 213 (Accession # NP_006519.1).

Predicted N-terminus: Asp 23

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.7 kDa. The protein migrates as 26-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

組織因子途徑抑制劑2（TFPI 2）也稱為胎盤蛋白5（PP5）和視網(wǎng)膜色素上皮細(xì)胞因子1（REF 1），是Kunitz型絲氨酸蛋白酶抑制劑家族的成員，是組織因子途徑抑制物（TFPI）的結(jié)構(gòu)同源物。TFPI2在足月胎盤中含量很高，在晚期妊娠時廣泛表達(dá)于臍靜脈內(nèi)皮細(xì)胞、肝臟、胎盤、心臟、胰腺和母體血清中。TFPI 2可能在調(diào)節(jié)纖溶酶介導(dǎo)的基質(zhì)重塑中發(fā)揮作用。抑制胰蛋白酶、纖溶酶、VIIa/組織因子和弱Xa因子，對凝血酶沒有影響。TFPI-2合成減少與許多病理生理過程有關(guān)，如炎癥、血管生成、動脈粥樣硬化、視網(wǎng)膜變性和腫瘤生長/轉(zhuǎn)移。