Human Fetuin A / FETUA Protein, His Tag 

分子別名（Synonym）

AHSG,FETUA,Fetuin A

表達區(qū)間及表達系統(tǒng)（Source）

Human Fetuin A, His Tag (FEA-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Val 367 (Accession # P02765-1).

Predicted N-terminus: Ala 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 38.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>98% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

α-2-HS-糖蛋白（AHSG）也稱為α-2-Z-球蛋白、Ba-α-2-糖蛋白、胎球蛋白-A（FETUA），是一種由A和B鏈兩個亞基組成的糖蛋白，屬于半胱氨酸蛋白酶抑制劑家族和胎球蛋白類血漿結(jié)合蛋白。AHSG/胎球蛋白A在胚胎細胞和成年肝細胞中高度表達，在單核細胞/巨噬細胞中表達程度較低。胎蛋白是白蛋白等載體蛋白。胎球蛋白-A/AHSG與鈣和磷酸鹽形成可溶性復(fù)合物，因此是不溶性磷酸鈣的載體。因此，AHSG/胎球蛋白A是一種有效的病理鈣化抑制劑。胎球蛋白A/FETUA促進凋亡細胞的吞噬作用，并在骨發(fā)育、鈣穩(wěn)態(tài)和胰島素敏感性中發(fā)揮作用。胎球蛋白-A缺乏的小鼠表現(xiàn)出軟組織的全身鈣化。