Glutaredoxin 1產(chǎn)品信息

英文名稱：Glutaredoxin 1

中文名稱：谷氧還蛋白

靶點(diǎn)別稱：GLRX1,GRX1

物種：Human

標(biāo)簽：His Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Glutaredoxin 1, His Tag (GLX-H5149) is expressed from E. coli cells. It contains AA Met 1 - Gln 106 (Accession # NP_001112362).

Predicted N-terminus: Met 1

蛋白結(jié)構(gòu)（Molecular Characterization） 

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 12.7 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

谷氧還蛋白-1（GRX1）也稱為硫轉(zhuǎn)移酶-1（TTase-1）、GLRX、GRX、GLRX1，屬于谷氧還肽家族。GRX1/GLRX含有一個(gè)谷氧還蛋白結(jié)構(gòu)域，它以還原或氧化的形式存在。GRX1/GRLX在NADPH和谷胱甘肽還原酶的存在下具有谷胱甘肽二硫化物氧化還原酶活性，并在酶聯(lián)核苷酸還原酶的谷胱甘肽依賴性脫氧核糖核苷酸合成中充當(dāng)電子載體。