NOTCH2產(chǎn)品信息

英文名稱：Neurogenic locus notch homolog protein 2

中文名稱：神經(jīng)源性基因座notch同源蛋白-2

靶點(diǎn)別稱：NOTCH2

物種：Human

標(biāo)簽：Fc Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human NOTCH2, Fc Tag (NO2-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Gln 530 (Accession # Q04721-1).

Predicted N-terminus: Leu 26

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 80.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

作為膜結(jié)合配體Jagged-1（JAG1）、Jagged-2（JAG2）和Delta-1（DLL1）的受體，調(diào)節(jié)細(xì)胞命運(yùn)的決定。當(dāng)配體通過釋放的缺口細(xì)胞內(nèi)結(jié)構(gòu)域（NICD）激活時，它與RBPJ/RBPSHH形成轉(zhuǎn)錄激活物復(fù)合物，并激活分裂位點(diǎn)增強(qiáng)子的基因。影響分化、增殖和凋亡程序的實施（按相似性）。參與骨重塑和穩(wěn)態(tài)。與RELA/p65合作，增強(qiáng)NFATc1啟動子活性，并積極調(diào)節(jié)RANKL誘導(dǎo)的破骨細(xì)胞分化。積極調(diào)節(jié)癌癥細(xì)胞的自我更新。