Stathmin 1產(chǎn)品信息

英文名稱：Stathmin

中文名稱：Stathmin蛋白

靶點(diǎn)別稱：STMN1,Stathmin 1,C1orf215,LAP18,OP18

物種：Human

標(biāo)簽：His Tag

屬性：Protein

標(biāo)記：Unconjugated

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Stathmin 1, His Tag (ST1-H5149) is expressed from E. coli cells. It contains AA Ala 2 - Asp 149 (Accession # NP_981946).

Predicted N-terminus: Met

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 18.1 kDa. The protein migrates as 18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Stathmin（STMN1）是Stathmin家族的成員，也被稱為白血病相關(guān)磷蛋白p18、癌蛋白18（Op18）、磷蛋白p19（pp19）、蛋白Pr22和pp17，其中含有一個(gè)SLD（Stathmin樣）結(jié)構(gòu)域。STMN1作為微管動力學(xué)的重要調(diào)節(jié)蛋白的功能已得到很好的表征。Stathmin（STMN1）與兩個(gè)二聚體α，β-微管蛋白分子相互作用，形成一個(gè)緊密的三元復(fù)合物，稱為T2S復(fù)合物。一摩爾STMN1通過stathmin樣結(jié)構(gòu)域（SLD）與兩摩爾微管蛋白二聚體結(jié)合。當(dāng)STMN1將微管蛋白螯合到T2S復(fù)合物中時(shí)，微管蛋白變得不可聚合。沒有微管蛋白聚合，就沒有微管組裝。STMN1還通過直接作用于微管末端來促進(jìn)微管解體。