Human IL-15 R alpha / CD215 Protein, Fc Tag (MALS verified)

分子別名（Synonym）

IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-15 R alpha, Fc Tag (ILA-H5253) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1).

Predicted N-terminus: Ile 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 44.8 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

IL15R是IL15RA、IL2RB和IL2RG的異三聚體。IL-15RA，也稱為CD215、IL-15Rα，是一種廣泛表達(dá)的60kDa跨膜糖蛋白。白細(xì)胞介素-15的高親和力受體?？梢皂樖胶头词叫盘?，其中來自一個(gè)細(xì)胞亞群的IL15R將IL15呈遞給相鄰的IL2RG表達(dá)細(xì)胞。不同異構(gòu)體的表達(dá)可能會改變或干擾信號轉(zhuǎn)導(dǎo)。白細(xì)胞介素（IL）-15超激動(dòng)劑/IL-15受體α融合復(fù)合物（IL-15SA/IL-15RA；ALT-803）激活CD8 T細(xì)胞和NK細(xì)胞上的IL-15受體，并顯示出顯著的抗腫瘤作用。信號轉(zhuǎn)導(dǎo)涉及SYK。與膜結(jié)合形式相比，可溶性sIL-15RA形式在所有腦結(jié)構(gòu)中都存在更高水平。