Human VEGFR2 / KDR Protein, His Tag (MALS verified)

分子別名（Synonym）

KDR,CD309,FLK1,VEGFR,VEGFR2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human VEGF R2, His Tag (KDR-H5227) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823.1).

Predicted N-terminus: Ala 20

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 85.2 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

VEGFR2也稱為CD309，是VEGFR的亞型之一。VEGF受體是血管內(nèi)皮生長(zhǎng)因子（VEGF）的受體。VEGFR有三種主要亞型，編號(hào)為1、2和3。VEGF受體具有由7個(gè)免疫球蛋白樣結(jié)構(gòu)域組成的細(xì)胞外部分、一個(gè)跨膜區(qū)和一個(gè)含有分裂酪氨酸激酶結(jié)構(gòu)域的細(xì)胞內(nèi)部分。VEGF-A與VEGFR-1（Flt-1）和VEGFR-2（KDR/Flk-1）結(jié)合。VEGFR-2似乎介導(dǎo)了幾乎所有已知的細(xì)胞對(duì)VEGF的反應(yīng)。VEGFR-1的功能不太明確，盡管它被認(rèn)為可以調(diào)節(jié)VEGFR-2信號(hào)傳導(dǎo)。VEGFR-1的另一個(gè)功能可能是充當(dāng)假受體/誘餌受體，將VEGF與VEGFR-2結(jié)合隔離開來（這在胚胎血管生成過程中似乎尤為重要）。此外，VEGFR2能夠在VEGF激活后與HIV-1細(xì)胞外Tat蛋白相互作用，似乎可以增強(qiáng)卡波濟(jì)氏肉瘤病變中的血管生成。