Mouse GDF-15 / MIC-1 Protein, His Tag

分子別名（Synonym）

GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse GDF-15, His Tag (GD5-M5149) is expressed from E. coli cells. It contains AA Ser 189 - Ala 303 (Accession # Q9Z0J7-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.6 kDa. The protein migrates as 16-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 0.085%TFA 30%ACN with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

生長分化因子15（GDF-15），也稱為巨噬細(xì)胞抑制性細(xì)胞因子1（MIC-1）。單核細(xì)胞中MIC-1 mRNA的表達(dá)受到與激活相關(guān)的各種刺激的上調(diào)，包括白細(xì)胞介素1β、腫瘤壞死因子α（TNF-α）、白細(xì)胞介蛋白2和巨噬細(xì)胞集落刺激因子，但不包括干擾素γ或脂多糖（LPS）。在正常和病理?xiàng)l件下，它在心肌細(xì)胞、脂肪細(xì)胞、巨噬細(xì)胞、內(nèi)皮細(xì)胞和血管平滑肌細(xì)胞中高度表達(dá)。GDF-15在組織損傷和炎癥狀態(tài)下會(huì)增加，并與心臟代謝風(fēng)險(xiǎn)有關(guān)。GDF-15水平升高與心血管疾病有關(guān)，如肥大、心力衰竭、動(dòng)脈粥樣硬化、內(nèi)皮功能障礙、肥胖、胰島素抵抗、糖尿病和糖尿病慢性腎病。GDF-15水平的升高與疾病的進(jìn)展和預(yù)后有關(guān)。