Mouse VEGFA Protein, Tag Free (HPLC-verified)

分子別名（Synonym）

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse VEGFA Protein, Tag Free (VE4-M4216) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # AAB22253.1 ).

Predicted N-terminus: Ala 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

Negative

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

血管內(nèi)皮生長因子A（VEGFA）也稱為血管通透性因子（VPF）。VEGFA屬于PDGF/VEGF生長因子家族。VEGFA是一種糖基化絲裂原，特異性作用于內(nèi)皮細(xì)胞，具有多種作用，包括介導(dǎo)血管通透性增加、誘導(dǎo)血管生成、血管生成和內(nèi)皮細(xì)胞生長、促進(jìn)細(xì)胞遷移和抑制凋亡?；蛘撸幋a自由分泌或細(xì)胞相關(guān)異構(gòu)體的剪接轉(zhuǎn)錄變體已被表征。由于選擇性剪接，VEGFA由一組三種主要亞型產(chǎn)生，如果產(chǎn)生任何三種亞型（VEGFA120、VEGFA164和VEGFA188），則不會導(dǎo)致小鼠血管缺陷和完全VEGFA敲除死亡。