PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein
表達區(qū)間及表達系統(tǒng)（Source）
PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (H2A-MP2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).
Predicted N-terminus: Gly 22 & Ile 21
蛋白結構（Molecular Characterization）
PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein is assembled by biotinylated monomer (H2A-M82E6) and PE-labeled streptavidin.
Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).
偶聯（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
卵清蛋白（OVA）可以基于特征明確的肽表位誘導體液和細胞免疫反應。OVA257-264八肽是首批被鑒定的OVA表位之一，其氨基酸序列為SIINFEKL，可被細胞毒性T淋巴細胞識別。如果卵清蛋白（Ova）通過皮諾體的滲透裂解從皮諾體釋放到細胞質中，則會被呈遞給T細胞。佐劑SIINFEKL肽免疫誘導小鼠長期CD8+T細胞免疫。
關鍵字： H-2Kb&B2M&OVA;SIINFEKL;四聚體蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產業(yè)領域提供關鍵生物試劑產品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下擁有品牌ACROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。