Human M-CSF / CSF-1 Protein, premium grade

分子別名（Synonym）

M-CSF,CSF-1,Lanimostim

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human M-CSF Protein, premium grade (MCF-H5218) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).

Predicted N-terminus: Glu 33

該產(chǎn)品是在我們嚴(yán)格的質(zhì)量控制體系下生產(chǎn)的，該體系包含一套全面的測試，包括無菌和內(nèi)毒素測試。在早期臨床前階段，產(chǎn)品性能經(jīng)過仔細(xì)驗(yàn)證和測試，以確保其與細(xì)胞培養(yǎng)用途或任何其他應(yīng)用的兼容性。當(dāng)準(zhǔn)備過渡到后期臨床階段時(shí)，我們還提供定制的GMP蛋白質(zhì)服務(wù)，以滿足您的需求。我們將與您合作，根據(jù)您的要求定制和開發(fā)GMP級產(chǎn)品，該產(chǎn)品也符合細(xì)胞療法細(xì)胞制造中使用的原材料和輔助材料的要求。

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 25.1 kDa. The protein migrates as 39 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

宿主蛋白殘留（Host Cell Protein）

<0.5 ng/μg of protein tested by ELISA.

宿主核酸殘留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

集落刺激因子1（CSF1），也稱為巨噬細(xì)胞集落刺激因素（M-CSF），是一種分泌的細(xì)胞因子，影響造血干細(xì)胞分化為巨噬細(xì)胞或其他相關(guān)細(xì)胞類型。真核細(xì)胞也產(chǎn)生M-CSF以對抗細(xì)胞間病毒感染。它是實(shí)驗(yàn)描述的三種集落刺激因子之一。M-CSF與集落刺激因子1受體結(jié)合。巨噬細(xì)胞集落刺激因子已被證明與PIK3R2相互作用。M-CSF（或CSF-1）是一種造血生長因子，參與單核細(xì)胞、巨噬細(xì)胞和骨髓祖細(xì)胞的增殖、分化和存活。血管壁中局部產(chǎn)生的M-CSF有助于動脈粥樣硬化的發(fā)展和進(jìn)展。