Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His Tag (active enzyme, MALS verified)
分子別名(Synonym)
CAIX,CA9,CA-IX,G250,MN,P54,58N,pMW1
表達區(qū)間及表達系統(tǒng)(Source)
Human Carbonic Anhydrase IX (38-414), His Tag (CA9-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).
Predicted N-terminus: Gln 38
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 41.7 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
碳酸酐酶(CA)是鋅金屬酶的一個大家族。CA形成一個酶家族,催化二氧化碳和水快速相互轉(zhuǎn)化為碳酸氫鹽和質(zhì)子(反之亦然),這是一種在沒有催化劑的情況下發(fā)生得相當緩慢的可逆反應。這種酶在動物體內(nèi)的功能之一是將二氧化碳和碳酸氫鹽相互轉(zhuǎn)化,以維持血液和其他組織中的酸堿平衡,并幫助將二氧化碳運出組織。大多數(shù)碳酸酐酶的活性位點都含有鋅離子。至少有五個不同的CA家族(α、β、γ、δ和ε)。
碳酸酐酶9(CA9/CAIX)也稱為膜抗原MN(MN)、腎細胞癌相關(guān)抗原G250,屬于α-碳酸酐酶家族。CA9/CAIX在pH 6.49時具有最佳活性。二氧化碳的可逆水合作用。CA IX參與pH調(diào)節(jié)。CA9可能參與細胞增殖和轉(zhuǎn)化的控制。CA-IX似乎是宮頸腫瘤的一種新型特異性生物標志物。
關(guān)鍵字: CAIX;CAIX蛋白;碳酸酐酶IX;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。