硫酸博來(lái)霉素|T6116

價(jià)格	￥287	￥397	￥672
包裝	1mg	2mg	5mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-09-14

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產(chǎn)品詳情

中文名稱:硫酸博來(lái)霉素	英文名稱:Bleomycin Sulfate
CAS:9041-93-4	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: ≥98%	產(chǎn)品類別: 抑制劑
貨號(hào): T6116

Product Introduction

Bioactivity

名稱	Bleomycin Sulfate
描述	Bleomycin Sulfate (Blenoxane) is a glycopeptide antibiotic, an inhibitor of DNA synthesis. Bleomycin Sulfate causes DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has antitumor activity.
細(xì)胞實(shí)驗(yàn)	ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)
體外活性	方法：人胰腺細(xì)胞 (PCCs) AsPC-1 和 MIA PaCa-2 用 Bleomycin Sulfate (0.1-100 μM) 處理 24-72 h，使用 MTT 方法檢測(cè)細(xì)胞生長(zhǎng)抑制情況。結(jié)果：AsPC-1 細(xì)胞對(duì) Bleomycin Sulfate 無(wú)反應(yīng)。Bleomycin Sulfate 顯著抑制了 MIA-PaCa-2 的細(xì)胞生長(zhǎng)，在處理 24/48/72 h 時(shí)的 IC50 分別為 5.9/6.4/2.6 μM。[1] 方法：間充質(zhì)干細(xì)胞 MSCs 用 Bleomycin Sulfate (1800 ng/mL) 處理 24-96 h，使用 Flow Cytometry 方法檢測(cè) sub-G1 和 caspase-3。結(jié)果：Bleomycin Sulfate 誘導(dǎo) sub-G1 和 caspase-3 的激活上調(diào)，表明 Bleomycin Sulfate 誘導(dǎo)細(xì)胞凋亡。[2] 方法：正常大鼠肺泡巨噬細(xì)胞用 Bleomycin Sulfate (0.01-1 μg/mL) 孵育 18 h，使用 MDGF Assay 測(cè)定其水平。結(jié)果：Bleomycin Sulfate 刺激巨噬細(xì)胞產(chǎn)生成纖維細(xì)胞生長(zhǎng)因子 (MDGF)。[3]
體內(nèi)活性	方法：為檢測(cè)體內(nèi)抗腫瘤活性，將 Bleomycin Sulfate (20 mg/kg) 腹腔注射給攜帶小鼠結(jié)直腸癌瘤腫瘤 CT26 shControl 或 shCRT 的 BALB/c 小鼠，每?jī)商煲淮?，給藥五次。結(jié)果：Bleomycin Sulfate 延遲了對(duì)照 shRNA 轉(zhuǎn)染的 CT26 細(xì)胞的生長(zhǎng)，但對(duì) CRT shRNA 轉(zhuǎn)染 CT26 細(xì)胞沒(méi)有影響。[4] 方法：為研究博來(lái)霉素誘導(dǎo)的小鼠肺纖維化的時(shí)間過(guò)程，將 Bleomycin Sulfate (0.06 mg/只 in 0.9% saline) 單次氣管內(nèi)滴注 (IT) 給 C57Bl/6J 小鼠。結(jié)果：Bleomycin Sulfate 誘導(dǎo)小鼠肺纖維化。該模型中評(píng)估肺纖維化的最合適的時(shí)間點(diǎn)是在 IT 滴注 Bleomycin Sulfate 后 14 天，這是基于觀察到的動(dòng)物在 14 天時(shí)出現(xiàn)廣泛的纖維化，但纖維化反應(yīng)的變異性較小，死亡率低于21天。[5]
存儲(chǔ)條件	keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : < 1 mg/mL (insoluble or slightly soluble) H2O : 92 mg/mL (60.8 mM) DMSO : 93 mg/mL (61.5 mM)
關(guān)鍵字	inhibit \| NSC 125066 \| DNA/RNA Synthesis \| Antibiotic \| Bleomycin \| NSC-125066 \| Inhibitor \| Bleomycin Sulfate
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關(guān)鍵字：硫酸博萊霉素|||硫酸博來(lái)霉素|||NSC125066|||Blenoxane|TargetMol

公司簡(jiǎn)介

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