Human FOLR3 Protein, His Tag (MALS verified)

分子別名（Synonym）

folate receptor 3 (gamma),Folate receptor 3,folate receptor gamma,FOLR3,FR-G,FR-gamma,gamma-Hfr,

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human FOLR3, His Tag (FO3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 245 (Accession # P41439-1).

Predicted N-terminus: Gln 23

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 27.4 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

FOLR3，也稱為葉酸受體γ，是FR的成員。葉酸及其還原衍生物通過兩種廣泛表達(dá)的轉(zhuǎn)運(yùn)蛋白進(jìn)行轉(zhuǎn)運(yùn)，即還原葉酸載體（RFC）和質(zhì)子偶聯(lián)葉酸轉(zhuǎn)運(yùn)蛋白（PCFT），以及通過一個(gè)被稱為葉酸受體（FR）的糖基磷脂酰肌醇（GPI）錨定受體家族進(jìn)行轉(zhuǎn)運(yùn)。葉酸受體（FR）是通過內(nèi)吞作用運(yùn)輸葉酸的高親和力受體。人類FR存在三種亞型，α和β帶有GPI錨，γ沒有GPI錨。