產(chǎn)品簡介

RFect小核酸轉(zhuǎn)染試劑是知名科學(xué)家崔坤元博士領(lǐng)導(dǎo)我公司研發(fā)團(tuán)隊(duì)在美國西雅圖實(shí)驗(yàn)室研發(fā)成功的一種新型的小核酸轉(zhuǎn)染試劑。RFect可用來轉(zhuǎn)染siRNA、miRNA mimics、miRNA inhibitor 等200bp以內(nèi)的小分子RNA，轉(zhuǎn)染細(xì)胞包括絕大多數(shù)貼壁生長的細(xì)胞，如一般細(xì)胞株、腫瘤細(xì)胞株等。目前，無論國外還是國內(nèi)，轉(zhuǎn)染試劑的主要成分均為脂質(zhì)體或聚乙烯亞胺(Polyethylenimine, PEI)，這兩種成分都具有很大的細(xì)胞毒性，并且轉(zhuǎn)染效果不好。RFect采用新型的動(dòng)物源性的納米材料，毒性很低并且擁有非常高的轉(zhuǎn)染性能。與其它品牌的小核酸轉(zhuǎn)染試劑相比，RFect的細(xì)胞轉(zhuǎn)染陽性率一般在90%以上，Lamin A/C 基因抑制效率在95%以上，而其它品牌轉(zhuǎn)染試劑的細(xì)胞轉(zhuǎn)染陽性率一般不超過70%，Lamin A/C 基因抑制效率不超過75%。RFect細(xì)胞毒性很低，轉(zhuǎn)染細(xì)胞死亡率不到10%，而其它品牌試劑的轉(zhuǎn)染細(xì)胞死亡率一般在30%以上。血清對轉(zhuǎn)染效果沒有影響，不必刻意添加或更換培養(yǎng)液。本產(chǎn)品適用于細(xì)胞株轉(zhuǎn)染，原代細(xì)胞轉(zhuǎn)染，請選擇RFectPM小核酸轉(zhuǎn)染試劑。

產(chǎn)品特點(diǎn)

·細(xì)胞轉(zhuǎn)染性能：細(xì)胞轉(zhuǎn)染陽性率高達(dá)90%以上，基因敲除效果明顯，A549細(xì)胞Lamin A/C 基因敲除效率在95%以上；

· 極低的細(xì)胞毒性：轉(zhuǎn)染細(xì)胞死亡率不到10%，大大降低了因細(xì)胞毒性對實(shí)驗(yàn)結(jié)果的影響；

· 轉(zhuǎn)染細(xì)胞范圍廣，絕大多數(shù)貼壁細(xì)胞株都能獲得比較理想的轉(zhuǎn)染結(jié)果。

Fig. 1. RFect sRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2. RFect siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig..3.RFect siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect siRNA transfection reagent. RFect concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.

適用細(xì)胞

293T（人腎上皮細(xì)胞），A549（人肺癌細(xì)胞），HEK 293（人胚腎細(xì)胞），HeLa（人宮頸癌細(xì)胞），MCF-7（人乳腺癌細(xì)胞），HepG2（人肝腫瘤細(xì)胞），HT-1080(人纖維肉瘤），HLF-a（人肺細(xì)胞），Huh-7（人肝癌細(xì)胞），KB（人口腔表皮樣癌細(xì)胞），PC3（人前列腺癌細(xì)胞），BRL-3A（大鼠正常肝細(xì)胞），DU 145（人前列腺癌細(xì)胞），Hepa 1-6（小鼠肝癌細(xì)胞），OVCAR3（人卵巢癌細(xì)胞），NCaP-FGC（人前列腺癌細(xì)胞），C2C12（成肌細(xì)胞），MDCK（犬腎細(xì)胞），CHO-K1，islk.219細(xì)胞（人肺癌細(xì)胞），HT-29（人結(jié)腸癌細(xì)胞），NCI-H1688（人典型小細(xì)胞肺腫瘤/癌細(xì)胞），RKO結(jié)腸癌細(xì)胞，Vero（非洲綠猴腎細(xì)胞），COS-1（非洲綠猴腎成纖維細(xì)胞），COS7-L（非洲綠猴腎成纖維細(xì)胞），COS-7（非洲綠猴腎成纖維細(xì)胞），SK-BR3（人乳腺癌細(xì)胞），Hepa 1cLc7（鼠肝癌細(xì)胞），Daoy（人髓母細(xì)胞瘤細(xì)胞），DBTRG-05MG（人腦膠質(zhì)母細(xì)胞瘤細(xì)胞），KLN 205（鼠肺磷癌細(xì)胞），Neuro-2a（小鼠神經(jīng)瘤母細(xì)胞），DI-TNC1（大鼠腦間質(zhì)細(xì)胞）等。