Human HE4 / WFDC2 Protein, His Tag

分子別名（Synonym）

HE4,WAP5,WFDC2,EDDM4

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human HE4, His Tag (HE4-H5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Phe 124 (Accession # AAH46106).

Predicted N-terminus: Glu 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 10.9 kDa. The protein migrates as 19-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

人附睪蛋白4（HE4）也稱為WAP四二硫鍵核心結(jié)構(gòu)域蛋白2（WFDC2）、附睪分泌蛋白E4、主要附睪特異性蛋白E4、推測(cè)蛋白酶抑制劑WAP5，是WFDC結(jié)構(gòu)域家族的成員。HE4/WFDC2在肺上皮細(xì)胞中表達(dá)，也在一些卵巢癌中表達(dá)。WFDC結(jié)構(gòu)域或WAP簽名基序包含八個(gè)半胱氨酸，在蛋白質(zhì)的核心形成四個(gè)二硫鍵，在許多家族成員中起蛋白酶抑制劑的作用。HE4也是一種小的分泌蛋白，可能參與精子成熟。