Biotinylated Human Sortilin / SORT1 Protein, His,Avitag? (MALS & SPR verified)

分子別名（Synonym）

Sortilin,SORT1,100 kDa NT receptor,Glycoprotein 95,Gp95,Gp95LDLCQ6,Neurotensin receptor 3,NT3NTR3,Ntr3,sortilin 1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human Sortilin Protein, His,Avitag (SON-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Asn 755 (Accession # Q99523-1).

Predicted N-terminus: Ser 78

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 79.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Sortilin是高爾基體隔室中的分選受體，也是細(xì)胞表面的清除受體。通過獨(dú)立于甘露糖-6-磷酸受體（M6PR）的途徑將蛋白質(zhì)從高爾基體運(yùn)輸?shù)饺苊阁w所需。溶酶體蛋白特異性結(jié)合高爾基體中的受體，產(chǎn)生的受體-配體復(fù)合物被轉(zhuǎn)運(yùn)到酸性前溶酶體室，在那里低pH值介導(dǎo)復(fù)合物的解離。然后，受體通過與逆轉(zhuǎn)錄酶的結(jié)合被再循環(huán)回高爾基體進(jìn)行另一輪運(yùn)輸。也是蛋白質(zhì)從高爾基體運(yùn)輸?shù)絻?nèi)體所必需的。通過介導(dǎo)促凋亡前體BDNF（proBDNF）和NGFB（proNGFB）的內(nèi)吞作用促進(jìn)神經(jīng)元凋亡。也充當(dāng)神經(jīng)降壓素的受體?？赡芡ㄟ^清除細(xì)胞外LPL促進(jìn)成骨分化過程中細(xì)胞外基質(zhì)的礦化。脂肪細(xì)胞可能需要形成含有葡萄糖轉(zhuǎn)運(yùn)蛋白SLC2A4/GLUT4的特殊儲存囊泡（GLUT4儲存囊泡或GSV）。這些囊泡提供了穩(wěn)定的SLC2A4池，并提高了對胰島素的反應(yīng)性。也可能介導(dǎo)從內(nèi)質(zhì)網(wǎng)到高爾基體的運(yùn)輸。